Skip Navigation

This Article
Right arrow Full Text Freely available
Right arrow Print PDF (429K) Freely available
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (9)
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Tanaka, H.
Right arrow Articles by Yuasa, Y.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Tanaka, H.
Right arrow Articles by Yuasa, Y.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Nucleic Acids Research, 2002, Vol. 30, No. 21 4728-4739
© 2002 Oxford University Press

The fission yeast pfh1+ gene encodes an essential 5' to 3' DNA helicase required for the completion of S-phase

Hiroyuki Tanaka*,1,2, Gi-Hyuck Ryu3, Yeon-Soo Seo3, Koichi Tanaka4, Hiroto Okayama4, Stuart A. MacNeill1 and Yasuhito Yuasa2

1 Wellcome Trust Centre for Cell Biology, University of Edinburgh, Michael Swann Building, King’s Buildings, Mayfield Road, Edinburgh EH9 3JR, UK, 2 Department of Molecular Oncology, Graduate School of Medicine and Dentistry, Tokyo Medical and Dental University, 1-5-45 Yushima Bunkyo-ku, Tokyo 113-8519, Japan, 3 National Creative Research Initiative Center for Cell Cycle Control, Samsung Biomedical Research Institute, Sungkyunkwan University School of Medicine, 300 Chunchun-Dong, Changan-Ku, Suwon-Si, Kyunggi-Do, 440-746, South Korea and 4 Department of Biochemistry and Molecular Biology, Graduate School of Medicine, The University of Tokyo, 7-3-1 Hongo Bunkyo-Ku, Tokyo 113-0033, Japan

*To whom correspondence should be addressed at Wellcome Trust Centre for Cell Biology, University of Edinburgh, Michael Swann Building, King’s Buildings, Mayfield Road, Edinburgh EH9 3JR, UK. Tel: +44 131 650 7085; Fax: +44 131 650 8650; Email: hiroyuki.tanaka{at}ed.ac.uk
Present address:
Koichi Tanaka, Research Institute of Molecular Pathology, Dr Bohr-Gasse 7, A-1030, Vienna, Austria

The Cdc24 protein plays an essential role in chromosomal DNA replication in the fission yeast Schizosaccharomyces pombe, most likely via its direct interaction with Dna2, a conserved endonuclease–helicase protein required for Okazaki fragment processing. To gain insights into Cdc24 function, we isolated cold-sensitive chromosomal suppressors of the temperature-sensitive cdc24-M38 allele. One of the complementation groups of such suppressors defined a novel gene, pfh1+, encoding an 805 amino acid nuclear protein highly homologous to the Saccharomyces cerevisiae Pif1p and Rrm3p DNA helicase family proteins. The purified Pfh1 protein displayed single-stranded DNA-dependent ATPase activity as well as 5' to 3' DNA helicase activity in vitro. Reverse genetic analysis in S.pombe showed that helicase activity was essential for the function of the Pfh1 protein in vivo. Schizosaccharomyces pombe cells carrying the cold-sensitive pfh1-R20 allele underwent cell cycle arrest in late S/G2-phase of the cell cycle when shifted to the restrictive temperature. This arrest was dependent upon the presence of a functional late S/G2 DNA damage checkpoint, suggesting that Pfh1 is required for the comple tion of DNA replication. Furthermore, at their permissive temperature pfh1-R20 cells were highly sensitive to the DNA-alkylating agent methyl methanesulphonate, implying a further role for Pfh1 in the repair of DNA damage.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Nucleic Acids ResHome page
J.-B. Boule and V. A. Zakian
Roles of Pif1-like helicases in the maintenance of genomic stability
Nucleic Acids Res., September 10, 2006; 34(15): 4147 - 4153.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
M. E. Budd, C. C. Reis, S. Smith, K. Myung, and J. L. Campbell
Evidence Suggesting that Pif1 Helicase Functions in DNA Replication with the Dna2 Helicase/Nuclease and DNA Polymerase {delta}.
Mol. Cell. Biol., April 1, 2006; 26(7): 2490 - 2500.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
J. Kim, K. Robertson, K. J. L. Mylonas, F. C. Gray, I. Charapitsa, and S. A. MacNeill
Contrasting effects of Elg1-RFC and Ctf18-RFC inactivation in the absence of fully functional RFC in fission yeast
Nucleic Acids Res., July 21, 2005; 33(13): 4078 - 4089.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
X. Peng, R. K. M. Karuturi, L. D. Miller, K. Lin, Y. Jia, P. Kondu, L. Wang, L.-S. Wong, E. T. Liu, M. K. Balasubramanian, et al.
Identification of Cell Cycle-regulated Genes in Fission Yeast
Mol. Biol. Cell, March 1, 2005; 16(3): 1026 - 1042.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
H. Tanaka, G.-H. Ryu, Y.-S. Seo, and S. A. MacNeill
Genetics of lagging strand DNA synthesis and maturation in fission yeast: suppression analysis links the Dna2-Cdc24 complex to DNA polymerase {delta}
Nucleic Acids Res., December 2, 2004; 32(21): 6367 - 6377.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
K. Tomita, T. Kibe, H.-Y. Kang, Y.-S. Seo, M. Uritani, T. Ushimaru, and M. Ueno
Fission Yeast Dna2 Is Required for Generation of the Telomeric Single-Strand Overhang
Mol. Cell. Biol., November 1, 2004; 24(21): 9557 - 9567.
[Abstract] [Full Text] [PDF]

Home page
 GeneticsHome page
J. B. Bessler and V. A. Zakian
The Amino Terminus of the Saccharomyces cerevisiae DNA Helicase Rrm3p Modulates Protein Function Altering Replication and Checkpoint Activity
Genetics, November 1, 2004; 168(3): 1205 - 1218.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
G.-H. Ryu, H. Tanaka, D.-H. Kim, J.-H. Kim, S.-H. Bae, Y.-N. Kwon, J. S. Rhee, S. A. MacNeill, and Y.-S. Seo
Genetic and biochemical analyses of Pfh1 DNA helicase function in fission yeast
Nucleic Acids Res., August 9, 2004; 32(14): 4205 - 4216.
[Abstract] [Full Text] [PDF]


Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.