Skip Navigation

This Article
Right arrow Full Text Freely available
Right arrow Print PDF (234K) Freely available
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (6)
Right arrowRequest Permissions
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Hwang, M.-S.
Right arrow Articles by Yoo, M.-A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Hwang, M.-S.
Right arrow Articles by Yoo, M.-A.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Nucleic Acids Research, 2002, Vol. 30, No. 23 5029-5035
© 2002 Oxford University Press

The Caudal homeodomain protein activates Drosophila E2F gene expression

Mi-Sun Hwang, Young-Shin Kim1, Na-Hyun Choi, Jae-Hong Park, Eun-Jin Oh, Eun-Jeong Kwon2,3, Masamitsu Yamaguchi2 and Mi-Ae Yoo*

Department of Molecular Biology and 1 Research Institute of Genetic Engineering, Pusan National University, Pusan 609-735, Korea, 2 Division of Biotechnology, Faculty of Textile Science, Kyoto Institute of Technology, Matsugasaki, Sakyo-ku, Kyoto 606-8585, Japan and 3 Division of Biological Science, Graduate School of Science, Nagoya University, Chikusa-ku, Nagoya 464-8602, Japan

*To whom correspondence should be addressed. Tel: +82 51 510 2278; Fax: +82 51 513 9258; Email: mayoo{at}pusan.ac.kr

The Drosophila caudal homeobox gene is required for definition of the anteroposterior axis and for gut development, and CDX1 and CDX2, human homologs, play crucial roles in the regulation of cell proliferation and differentiation in the intestine. Most studies have indicated tumor suppressor functions of Cdx2, with inhibition of proliferation, while the effects of Cdx1 are more controversial. The influence of Drosophila Caudal on cell proliferation is unknown. In this study, we found three potential Caudal binding sequences in the 5'-flanking region of the Drosophila E2F (DE2F) gene and showed by transient transfection assays that they are involved in Caudal transactivation of the dE2F gene promoter. Analyses with transgenic flies carrying an E2F-lacZ fusion gene, with and without mutation in the Caudal binding site, indicated that the Caudal binding sites are required for expression of dE2F in living flies. Caudal-induced E2F expression was also confirmed with a GAL4-UAS system in living flies. In addition, ectopic expression of Caudal with heat-shock promotion induced melanotic tumors in larvae. These results suggest that Caudal is involved in regulation of proliferation through transactivation of the E2F gene in Drosophila.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 GENES CELLSHome page
K. Legent, A. Dutriaux, R. Delanoue, and J. Silber
Cell cycle genes regulate vestigial and scalloped to ensure normal proliferation in the wing disc of Drosophila melanogaster.
Genes Cells, August 1, 2006; 11(8): 907 - 918.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
Y.-J. Choi, T.-Y. Choi, M. Yamaguchi, A. Matsukage, Y.-S. Kim, and M.-A. Yoo
Transcriptional regulation of the Drosophila caudal homeobox gene by DRE/DREF
Nucleic Acids Res., July 14, 2004; 32(12): 3734 - 3742.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.