Nucleic Acids Research, 2002, Vol. 30, No. 23 e134
© 2002 Oxford University Press
Stringent doxycycline dependent control of CRE recombinase in vivo
Zentrum für Molekulare Biologie der Universität Heidelberg (ZMBH), Im Neuenheimer Feld 282, D-69120 Heidelberg, Germany and 1 Institute for Genetics, University of Cologne, Weyertal 121, D-50931 Cologne, Germany
*To whom correspondence should be addressed. Tel: +49 6221 548214; Fax: +49 6221 545892; Email: h.bujard{at}zmbh.uni-heidelberg.de
Present addresses:
Frieder Schwenk, Artemis Pharmaceuticals GmbH, Neurather Ring 1, D-51063 Cologne, Germany
Klaus Rajewsky, Center for Blood Research, Harvard University, 200 Longwood Ave, Boston, MA 02115, USA
The authors wish it to be known that, in their opinion, the first two authors should be regarded as joint First Authors
The strategy of modulating gene activities in vivo via CRE/loxP recombination would greatly profit from subjecting the recombination event to an independent and stringent temporal control. Here, we describe a transgenic mouse line, LC-1, where the expression of the cre and luciferase gene is tightly controlled by the Tet system. Using the R26R mouse line as indicator for CRE activity, and mouse lines expressing tetracycline controlled transactivators (tTA/rtTA) in various tissues, we show that; (i) in the non-induced state CRE recombinase is tightly controlled throughout the development and adulthood of an animal; (ii) upon induction, efficient recombination occurs in the adult animal in all tissues where tTA/rtTA is present, including hepatocytes, kidney cells, neurons and T lymphocytes; and (iii) no position effect appears to be caused by the LC-1 locus. Moreover, using the novel rTALAP-1 mouse line, we show that in hepatocytes, complete deletion of the loxP-flanked insert in R26R animals is achieved less than 48 h after induction. Thus, the LC-1 mouse appears suitable for exploiting two rapidly increasing collections of mouse lines of which one provides tTA/rtTA in specific cell types/tissues, and the other a variety of loxP-flanked genes.
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  I. Weidenfeld, M. Gossen, R. Low, D. Kentner, S. Berger, D. Gorlich, D. Bartsch, H. Bujard, and K. Schonig Inducible expression of coding and inhibitory RNAs from retargetable genomic loci Nucleic Acids Res., April 1, 2009; 37(7): e50 - e50. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. Appel, D. B. Kershaw, B. Smeets, G. Yuan, A. Fuss, B. Frye, M. Elger, W. Kriz, J. Floege, and M. J. Moeller Recruitment of Podocytes from Glomerular Parietal Epithelial Cells J. Am. Soc. Nephrol., February 1, 2009; 20(2): 333 - 343. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Pich, S. Humme, M.-P. Spindler, A. Schepers, and W. Hammerschmidt Conditional gene vectors regulated in cis Nucleic Acids Res., August 1, 2008; 36(13): e83 - e83. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Varshavsky From the Cover: Targeting the absence: Homozygous DNA deletions as immutable signposts for cancer therapy PNAS, September 18, 2007; 104(38): 14935 - 14940. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Zhang, Y. Liu, C. Beard, D. A. Tuveson, R. Jaenisch, T. E. Jacks, and H. F. Lodish Expression of oncogenic K-ras from its endogenous promoter leads to a partial block of erythroid differentiation and hyperactivation of cytokine-dependent signaling pathways Blood, June 15, 2007; 109(12): 5238 - 5241. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. A. Davey and H. E. MacLean Current and future approaches using genetically modified mice in endocrine research Am J Physiol Endocrinol Metab, September 1, 2006; 291(3): E429 - E438. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Bockamp, C. Antunes, M. Maringer, R. Heck, K. Presser, S. Beilke, S. Ohngemach, R. Alt, M. Cross, R. Sprengel, et al. Tetracycline-controlled transgenic targeting from the SCL locus directs conditional expression to erythrocytes, megakaryocytes, granulocytes, and c-kit-expressing lineage-negative hematopoietic cells Blood, September 1, 2006; 108(5): 1533 - 1541. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Viatte, G. Nicolas, D.-Q. Lou, M. Bennoun, J.-C. Lesbordes-Brion, F. Canonne-Hergaux, K. Schonig, H. Bujard, A. Kahn, N. C. Andrews, et al. Chronic hepcidin induction causes hyposideremia and alters the pattern of cellular iron accumulation in hemochromatotic mice Blood, April 1, 2006; 107(7): 2952 - 2958. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. P. Biju, Y. Akai, N. Shrimanker, and V. H. Haase Protection of HIF-1-deficient primary renal tubular epithelial cells from hypoxia-induced cell death is glucose dependent Am J Physiol Renal Physiol, December 1, 2005; 289(6): F1217 - F1226. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. Plum, F. T. Wunderlich, S. Baudler, W. Krone, and J. C. Bruning Transgenic and Knockout Mice in Diabetes Research: Novel Insights into Pathophysiology, Limitations, and Perspectives Physiology, June 1, 2005; 20(3): 152 - 161. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. F. Higgins, M. P. Biju, Y. Akai, A. Wutz, R. S. Johnson, and V. H. Haase Hypoxic induction of Ctgf is directly mediated by Hif-1 Am J Physiol Renal Physiol, December 1, 2004; 287(6): F1223 - F1232. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. E. Krestel, D. R. Shimshek, V. Jensen, T. Nevian, J. Kim, Y. Geng, T. Bast, A. Depaulis, K. Schonig, F. Schwenk, et al. A Genetic Switch for Epilepsy in Adult Mice J. Neurosci., November 17, 2004; 24(46): 10568 - 10578. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Kemp, H. Ireland, E. Clayton, C. Houghton, L. Howard, and D. J. Winton Elimination of background recombination: somatic induction of Cre by combined transcriptional regulation and hormone binding affinity Nucleic Acids Res., July 1, 2004; 32(11): e92 - e92. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. R. Gallagher, K. Schonig, N. Brown, H. Bujard, and R. Witzgall Use of the Tetracycline System for Inducible Protein Synthesis in the Kidney J. Am. Soc. Nephrol., August 1, 2003; 14(8): 2042 - 2051. [Abstract] [Full Text] [PDF]
|
|