Nucleic Acids Research, 2002, Vol. 30, No. 24 5476-5484
© 2002 Oxford University Press
Mutations in U5 snRNA loop 1 influence the splicing of different genes in vivo
School of Biological Sciences, University of Manchester, Oxford Road, Manchester M13 9PT, UK
*Tel: +44 161 275 7393; Fax: +44 161 275 5082; Email: rokeefe{at}man.ac.uk
The U5 snRNA loop 1 is characterized by the conserved sequence G1C2C3U4U5U6Y7A8Y9 and is essential for the alignment of exons during the second step of pre-mRNA splicing in Saccharo myces cerevisiae. Despite this sequence conservation the size, rather than sequence, of loop 1 is critical for exon alignment in vitro. To determine the in vivo requirements for U5 loop 1 a library of loop 1 sequences was transformed into a yeast strain where the endogenous U5 gene was deleted. Comparison of viable mutations in loop 1 revealed that position 6 was invariant and positions 5 and 7 displayed some sequence conservation. These data indicate positions 5, 6 and 7 in loop 1 are important for U5 function in vivo. A screen for mutations that suppress the temperature-sensitive phenotype of three loop 1 mutants produced eight intragenic suppressors all containing alterations in loop 1. Further analysis of these temperature-sensitive mutants revealed that each displayed distinct cell cycle arrest phenotypes and pre-mRNA splicing inhibition patterns. The cell cycle arrest is likely attributed to inefficient splicing of 
-tubulin pre-mRNA in one mutant and actin pre-mRNA in another. These results suggest that various mutations in loop 1 may affect the splicing of different pre-mRNAs in vivo.
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  L. N. Frazer, S. C. Lovell, and R. T. O'Keefe Analysis of Synthetic Lethality Reveals Genetic Interactions Between the GTPase Snu114p and snRNAs in the Catalytic Core of the Saccharomyces cerevisiae Spliceosome Genetics, October 1, 2009; 183(2): 497 - 515. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. J. Kershaw, J. D. Barrass, J. D. Beggs, and R. T. O'Keefe Mutations in the U5 snRNA result in altered splicing of subsets of pre-mRNAs and reduced stability of Prp8 RNA, July 1, 2009; 15(7): 1292 - 1304. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Aronova, D. Bacikova, L. B. Crotti, D. S. Horowitz, and B. Schwer Functional interactions between Prp8, Prp18, Slu7, and U5 snRNA during the second step of pre-mRNA splicing RNA, September 1, 2007; 13(9): 1437 - 1444. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. J. GRAINGER and J. D. BEGGS Prp8 protein: At the heart of the spliceosome RNA, May 1, 2005; 11(5): 533 - 557. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. Bacikova and D. S. Horowitz Genetic and Functional Interaction of Evolutionarily Conserved Regions of the Prp18 Protein and the U5 snRNA Mol. Cell. Biol., March 15, 2005; 25(6): 2107 - 2116. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. J. Silverman, A. Maeda, J. Wei, P. Smith, J. D. Beggs, and R.-J. Lin Interaction between a G-Patch Protein and a Spliceosomal DEXD/H-Box ATPase That Is Critical for Splicing Mol. Cell. Biol., December 1, 2004; 24(23): 10101 - 10110. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. C. DOBBYN and R. T. O'KEEFE Analysis of Snu13p mutations reveals differential interactions with the U4 snRNA and U3 snoRNA RNA, February 1, 2004; 10(2): 308 - 320. [Abstract] [Full Text] [PDF]
|
|