Skip Navigation

This Article
Right arrow Full Text Freely available
Right arrow Print PDF (248K) Freely available
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Salin, H.
Right arrow Articles by Dumas, S.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Salin, H.
Right arrow Articles by Dumas, S.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Nucleic Acids Research, 2002, Vol. 30, No. 4 e17
© 2002 Oxford University Press

A novel sensitive microarray approach for differential screening using probes labelled with two different radioelements

H. Salin, T. Vujasinovic, A. Mazurie, S. Maitrejean1, C. Menini, J. Mallet and S. Dumas*

LGN, UMR 7091, CNRS, Bâtiment CERVI, 5ème Étage, Hôpital Pitié Salpêtrière, 83 boulevard de l’Hôpital, F-75013 Paris, France and 1Biospace Mesures, 10 rue Mercoeur, F-75011 Paris, France

We have developed a novel microarray approach for differential screening using probes labelled with two different radioelements. The complementary DNAs from the reverse transcription of mRNAs from two different biological samples were labelled with radioelements of significantly different energies (3H and 35S or 33P). Radioactive images corresponding to the expressed genes were acquired with a MicroImager, a real time, high resolution digital autoradiography system. An algorithm was used to process the data such that the initially acquired radioactive image was filtered into two subimages, each representative of the hybridisation result specific for one probe. The simultaneous screening of gene expression in two different biological samples requires <100 ng mRNA without any amplification. In such conditions, the technique is sensitive enough to directly quantify the amount of mRNA even when present in small amounts: 107 molecules in the probe as assessed with an added control sequence and 2 x 105 molecules with an endogenous tyrosine hydroxylase mRNA. This novel technique of double radioactive labelling on a microarray is thus suitable for the comparison of gene expression in two different biological samples available in only small quantities. Consequently, it has great potential for various biological fields, such as neuroscience.

* To whom correspondence should be addressed. Tel: +33 1 42 17 75 56; Fax: +33 1 42 17 75 33; Email: sdumas{at}infobiogen.fr


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Nucleic Acids ResHome page
L. Petalidis, S. Bhattacharyya, G. A. Morris, V. P. Collins, T. C. Freeman, and P. A. Lyons
Global amplification of mRNA by template-switching PCR: linearity and application to microarray analysis
Nucleic Acids Res., November 15, 2003; 31(22): e142 - e142.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
A. M. Talaat, S. T. Howard, W. Hale IV, R. Lyons, H. Garner, and S. A. Johnston
Genomic DNA standards for gene expression profiling in Mycobacterium tuberculosis
Nucleic Acids Res., October 15, 2002; 30(20): e104 - e104.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.