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Nucleic Acids Research, 2002, Vol. 30, No. 5 1224-1232
© 2002 Oxford University Press

Homologous recombination is essential for RAD51 up-regulation in Saccharomyces cerevisiae following DNA crosslinking damage

Yuval Cohen, Michele Dardalhon and Dietrich Averbeck*

Institut Curie, Section de Recherche, UMR 2027 CNRS/IC, LRC-28V du CEA, Centre Universitaire Paris-Sud, Bât. 110, F-91405 Orsay, France

We have determined the kinetics of up-regulation of the homologous recombination gene RAD51, one of the genes induced following DNA damage in isogenic haploid DNA repair-deficient mutants of Saccharomyces cerevisiae, using treatment with the DNA crosslinking agent 8-methoxypsoralen. We show that RAD51 is up-regulated concomitantly, although independently, with a shift from the G1 cell cycle phase to G2/M arrest. This up-regulation is absent in homologous recombination repair-deficient mutants and increased in mutants deficient in nucleotide excision repair and pol{zeta}-dependent translesion synthesis. We demonstrate that the Rad53-dependent DNA damage signal transduction cascade is active in RAD51 non-inducing mutants. However, when independently eliminated, it too abolishes RAD51 up-regulation. We present a model in which RAD51 up-regulation requires two signals: one depending on the Rad53-dependent DNA damage signal transduction cascade and the other on homologous recombination repair.

* To whom correspondence should be addressed. Tel: +33 169 867 188; Fax: +33 169 869 429; Email: dietrich.averbeck{at}curie.u-psud.fr


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