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Nucleic Acids Research, 2002, Vol. 30, No. 6 1364-1370
© 2002 Oxford University Press

Methylation of adenine in the nuclear DNA of Tetrahymena is internucleosomal and independent of histone H1

Kathleen M. Karrer* and Teresa A. VanNuland

Department of Biology, Wehr Life Sciences, Marquette University, PO Box 1881, Milwaukee, WI 53201-1881, USA

There are about 50 copies of each chromosome in the somatic macronucleus of the ciliated protozoan Tetrahymena. Approximately 0.8% of the adenine residues in the macronuclear DNA of Tetrahymena are methylated to N6-methyladenine. The degree of methylation varies between sites from a very low percentage to >90%. In this study a correlation was found between nucleosome positioning and DNA methylation. Eight GATC sites with different levels of methylation were examined. There was a direct correlation between the degree of methylation and proximity to linker DNA at these sites. Although methylation occurs preferentially in linker DNA, the patterns and extent of methylation in a histone H1 knockout strain were virtually indistinguishable from those in wild-type cells.

* To whom correspondence should be addressed. Tel: +1 414 288 1474; Fax: +1 414 288 7357; Email: kathleen.karrer{at}marquette.edu Present address:Teresa A. VanNuland, Abbott Laboratories, 200 Abbott Park Road, Abbott Park, IL 60064-3537, USA +AF416723, L25254 and L34029


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