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Nucleic Acids Research, 2002, Vol. 30, No. 7 1585-1592
© 2002 Oxford University Press

A novel DNA polymerase homologous to Escherichia coli DNA polymerase I from a higher plant, rice (Oryza sativa L.)

Seisuke Kimura, Yukinobu Uchiyama, Nobuyuki Kasai, Satoshi Namekawa, Ai Saotome, Tadamasa Ueda1, Tsuyu Ando2, Toyotaka Ishibashi, Masahiko Oshige, Tomoyuki Furukawa, Taichi Yamamoto, Junji Hashimoto1,3 and Kengo Sakaguchi*

Department of Applied Biological Science, Faculty of Science and Technology, Tokyo University of Science, 2641 Yamazaki, Noda, Chiba 278-8510, Japan, 1National Institute of Agrobiological Sciences, Tsukuba , Ibaraki 305-0854, Japan, 2Institute of the Society for Techno-innovation of Agriculture, Forestry and Fisheries, Tsukuba, Ibaraki 305-0854, Japan and 3Core Research for Evolutional Science and Technology (CREST), Japan Science and Technology Corporation (JST), Ochanomizu, Chiyodaku, Tokyo101-0062, Japan

A novel DNA polymerase, designated as OsPolI-like, has been identified from the higher plant, rice (Oryza sativa L. cv. Nipponbare). The OsPolI-like cDNA was 3765 bp in length, and the open reading frame encoded a predicted product of 977 amino acid residues with a molecular weight of 100 kDa. The OsPolI-like gene has been mapped to chromosome 8 and contains 12 exons and 11 introns. The encoded protein showed a high degree of sequence and structural homology to Escherichia coli pol I protein, but differed from DNA polymerase {gamma} and {theta}. The DNA polymerase domain of OsPolI-like showed DNA polymerase activity. Subcellular fractionation analysis suggested that the protein is localized in the plastid. Northern and western blotting, and in situ hybridization analyses demonstrated preferential expression of OsPolI-like in meristematic tissues such as shoot apical meristem, root apical meristem, leaf primordia and the marginal meristem. Interestingly, no expression was detected in mature leaves, although they have a high chloroplast content. These properties indicated that OsPolI-like is a novel plant DNA polymerase. The function of OsPolI-like is discussed in relation to plastid maturation.

* To whom correspondence should be addressed. Tel: +81 471 24 1501; Fax: +81 471 23 9767; Email: kengo{at}rs.noda.sut.ac.jp


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