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Nucleic Acids Research, 2002, Vol. 30, No. 7 e33
© 2002 Oxford University Press

TspGWI, a thermophilic class-IIS restriction endonuclease from Thermus sp., recognizes novel asymmetric sequence 5'-ACGGA(N11/9)-3'

Agnieszka Zylicz-Stachula1, Renata I. Harasimowicz-Slowinska1, Ireneusz Sobolewski1 and Piotr M. Skowron1,2,*

1EURx Ltd, ul. Jaíkowa Dolina 29, 80-286 Gdaásk, Poland and 2Department of Microbiology, Technical University of Gdaásk, Gdaásk, Poland

A novel prototype class-IIS restriction endonuclease, TspGWI, was isolated from the thermophilic bacterium Thermus sp. GW. The recognition sequence and cleavage positions have been established: TspGWI recognizes the non-palindromic 5-bp sequence 5'-ACGGA-3' and cleaves the DNA 11 and 9 nt downstream in the top and bottom strand, respectively. In addition, an accompanying endonuclease, TspGWII, an isoschizomer of Pst I, was found in Thermus sp. GW cells.

* To whom correspondence should be addressed. Tel: +48 58 3452155; Fax: +48 58 3417423; Email: piotrs{at}eurx.com.pl


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