Skip Navigation

This Article
Right arrow Full Text Freely available
Right arrow Print PDF (327K) Freely available
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (17)
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Harata, M.
Right arrow Articles by Mochizuki, R.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Harata, M.
Right arrow Articles by Mochizuki, R.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Nucleic Acids Research, 2002, Vol. 30, No. 8 1743-1750
© 2002 Oxford University Press

Correlation between chromatin association and transcriptional regulation for the Act3p/Arp4 nuclear actin-related protein of Saccharomyces cerevisiae

Masahiko Harata*, Yan Zhang1, David J. Stillman1, Daisuke Matsui, Yukako Oma, Katsuhiko Nishimori and Ryo Mochizuki

Laboratory of Molecular Biology, Department of Molecular and Cell Biology, Division of Life Science, Graduate School of Agricultural Science, Tohoku University, Tsutsumidori-Amamiyamachi 1-1, Aoba-ku, Sendai 981-8555, Japan and 1Department of Pathology, University of Utah Health Sciences Center, 50 North Medical Drive, Room 5C124 SOM, Salt Lake City, UT 84132, USA

Actin-related proteins (Arps), which share a basal structure with actin but have distinct functions, have been found in a wide variety of organisms. While their functions are not yet clear, some Arps are localized in the nucleus and are suggested to contribute to the regulation of transcription. An essential gene of Saccharomyces cerevisiae, Act3p/Arp4, encodes the first identified nuclear Arp, which has been shown to bind to core histones in vitro. Here we have analyzed the in vivo function of Act3p/Arp4 on the his4-912{delta} promoter. Chromatin immunoprecipitation assays show that Act3p/Arp4 is bound to the entire his4-912{delta} promoter region. Conditional act3/arp4 mutations affect transcription from the his4-912{delta} promoter, where decreased Act3p/Arp4 binding and a change in nuclease sensitivity of chromatin were observed, showing the involvement of Act3p/Arp4 in the regulation of gene expression through the organization of chromatin structure. Taken together with the presence of Act3p/Arp4 in chromatin remodeling and histone acetyltransferase complexes, it is suggested that Act3p/Arp4 functions in transcriptional regulation to recruit chromatin remodeling and histone acetyltransferase complexes onto chromatin.

* To whom correspondence should be addressed. Tel: +81 22 717 8771; Fax: +81 22 717 8883; Email: mharata{at}biochem.tohoku.ac.jp Present address:Yan Zhang, Department of Microbiology and Molecular Genetics, UMDNJ–New Jersey Medical School, Newark, NJ 07103, USA


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Nucleic Acids ResHome page
H. Ogiwara, A. Ui, S. Kawashima, K. Kugou, F. Onoda, H. Iwahashi, M. Harata, K. Ohta, T. Enomoto, and M. Seki
Actin-related protein Arp4 functions in kinetochore assembly
Nucleic Acids Res., May 14, 2007; 35(9): 3109 - 3117.
[Abstract] [Full Text] [PDF]

Home page
 J BiochemHome page
F. Steinboeck, L. Krupanska, A. Bogusch, A. Kaufmann, and E. Heidenreich
Novel Regulatory Properties of Saccharomyces cerevisiae Arp4.
J. Biochem., April 1, 2006; 139(4): 741 - 751.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
R. B. Meagher, R. B. Deal, M. K. Kandasamy, and E. C. McKinney
Nuclear Actin-Related Proteins as Epigenetic Regulators of Development
Plant Physiology, December 1, 2005; 139(4): 1576 - 1585.
[Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
J. Muller, Y. Oma, L. Vallar, E. Friederich, O. Poch, and B. Winsor
Sequence and Comparative Genomic Analysis of Actin-related Proteins
Mol. Biol. Cell, December 1, 2005; 16(12): 5736 - 5748.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
A. Minoda, S. Saitoh, K. Takahashi, and T. Toda
BAF53/Arp4 Homolog Alp5 in Fission Yeast Is Required for Histone H4 Acetylation, Kinetochore-Spindle Attachment, and Gene Silencing at Centromere
Mol. Biol. Cell, January 1, 2005; 16(1): 316 - 327.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
M. Ueno, T. Murase, T. Kibe, N. Ohashi, K. Tomita, Y. Murakami, M. Uritani, T. Ushimaru, and M. Harata
Fission yeast Arp6 is required for telomere silencing, but functions independently of Swi6
Nucleic Acids Res., February 2, 2004; 32(2): 736 - 741.
[Abstract] [Full Text] [PDF]


Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.