Skip Navigation

This Article
Right arrow Full Text Freely available
Right arrow Print PDF (358K) Freely available
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (34)
Right arrowRequest Permissions
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Arhin, G. K.
Right arrow Articles by Wilusz, J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Arhin, G. K.
Right arrow Articles by Wilusz, J.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Nucleic Acids Research, 2002, Vol. 30, No. 8 1842-1850
© 2002 Oxford University Press

Downstream sequence elements with different affinities for the hnRNP H/H' protein influence the processing efficiency of mammalian polyadenylation signals

George K. Arhin, Monika Boots, Paramjeet S. Bagga1, Christine Milcarek2 and Jeffrey Wilusz*

Department of Microbiology and Molecular Genetics, UMDNJ–New Jersey Medical School, 185 South Orange Avenue, Newark, NJ 07103, USA, 1School of Theoretical and Applied Science, Ramapo College of New Jersey, 505 Ramapo Valley Road, Mahwah, NJ 07430, USA and 2Department of Molecular Genetics and Biochemistry, University of Pittsburgh School of Medicine, Pittsburgh, PA 15261, USA

Auxiliary factors likely play an important role in determining the polyadenylation efficiency of mammalian pre-mRNAs. We previously identified an auxiliary factor, hnRNP H/H', which stimulates 3'-end processing through an interaction with sequences downstream of the core elements of the SV40 late polyadenylation signal. Using in vitro reconstitution assays we have demonstrated that hnRNP H/H' can stimulate processing of two additional model polyadenylation signals by binding at similar relative downstream locations but with significantly different affinities. A short tract of G residues was determined to be a common property of all three hnRNP H/H' binding sites. A survey of mammalian polyadenylation signals identified potential G-rich hnRNP H/H' binding sites at similar downstream locations in ~34% of these signals. All of the novel G-rich elements tested were found to bind hnRNP H/H' protein and the processing of selected signals identified in the survey was stimulated by the protein both in vivo and in vitro. Downstream G-rich tracts, therefore, are a common auxiliary element in mammalian polyadenylation signals. Sequences capable of binding hnRNP H protein with varying affinities may play a role in determining the processing efficiency of a significant number of mammalian polyadenylation signals.

* To whom correspondence should be addressed. Tel: +1 973 972 5218; Fax: +1 973 972 3644; Email: wilusz{at}umdnj.edu


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Nucleic Acids ResHome page
S. Millevoi, A. Decorsiere, C. Loulergue, J. Iacovoni, S. Bernat, M. Antoniou, and S. Vagner
A physical and functional link between splicing factors promotes pre-mRNA 3' end processing
Nucleic Acids Res., June 8, 2009; (2009) gkp470v1.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
S. D. Creacy, E. D. Routh, F. Iwamoto, Y. Nagamine, S. A. Akman, and J. P. Vaughn
G4 Resolvase 1 Binds Both DNA and RNA Tetramolecular Quadruplex with High Affinity and Is the Major Source of Tetramolecular Quadruplex G4-DNA and G4-RNA Resolving Activity in HeLa Cell Lysates
J. Biol. Chem., December 12, 2008; 283(50): 34626 - 34634.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
O. Kikin, Z. Zappala, L. D'Antonio, and P. S. Bagga
GRSDB2 and GRS_UTRdb: databases of quadruplex forming G-rich sequences in pre-mRNAs and mRNAs
Nucleic Acids Res., January 11, 2008; 36(suppl_1): D141 - D148.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
J. Blechingberg, S. Lykke-Andersen, T. H. Jensen, A. L. Jorgensen, and A. L. Nielsen
Regulatory mechanisms for 3'-end alternative splicing and polyadenylation of the Glial Fibrillary Acidic Protein, GFAP, transcript
Nucleic Acids Res., December 3, 2007; 35(22): 7636 - 7650.
[Abstract] [Full Text] [PDF]

Home page
 RNAHome page
F. Guan, R. M. Caratozzolo, R. Goraczniak, E. S. Ho, and S. I. Gunderson
A bipartite U1 site represses U1A expression by synergizing with PIE to inhibit nuclear polyadenylation
RNA, December 1, 2007; 13(12): 2129 - 2140.
[Abstract] [Full Text] [PDF]

Home page
 J. Virol.Home page
N. L. Maciolek and M. T. McNally
Serine/Arginine-Rich Proteins Contribute to Negative Regulator of Splicing Element-Stimulated Polyadenylation in Rous Sarcoma Virus
J. Virol., October 15, 2007; 81(20): 11208 - 11217.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
M. Dalziel, N. M. Nunes, and A. Furger
Two G-Rich Regulatory Elements Located Adjacent to and 440 Nucleotides Downstream of the Core Poly(A) Site of the Intronless Melanocortin Receptor 1 Gene Are Critical for Efficient 3' End Processing
Mol. Cell. Biol., March 1, 2007; 27(5): 1568 - 1580.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
H. Zhu, H.-L. Zhou, R. A. Hasman, and H. Lou
Hu Proteins Regulate Polyadenylation by Blocking Sites Containing U-rich Sequences
J. Biol. Chem., January 26, 2007; 282(4): 2203 - 2210.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
D. Liu, J. M. Brockman, B. Dass, L. N. Hutchins, P. Singh, J. R. McCarrey, C. C. MacDonald, and J. H. Graber
Systematic variation in mRNA 3'-processing signals during mouse spermatogenesis
Nucleic Acids Res., January 12, 2007; 35(1): 234 - 246.
[Abstract] [Full Text] [PDF]

Home page
 J. Virol.Home page
J. E. Wilusz and K. L. Beemon
The Negative Regulator of Splicing Element of Rous Sarcoma Virus Promotes Polyadenylation
J. Virol., October 1, 2006; 80(19): 9634 - 9640.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
C. Dominguez and F. H.-T. Allain
NMR structure of the three quasi RNA recognition motifs (qRRMs) of human hnRNP F and interaction studies with Bcl-x G-tract RNA: a novel mode of RNA recognition
Nucleic Acids Res., August 2, 2006; 34(13): 3634 - 3645.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
O. Kikin, L. D'Antonio, and P. S Bagga
QGRS Mapper: a web-based server for predicting G-quadruplexes in nucleotide sequences.
Nucleic Acids Res., July 1, 2006; 34(Web Server issue): W676 - W682.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
R. Kostadinov, N. Malhotra, M. Viotti, R. Shine, L. D'Antonio, and P. Bagga
GRSDB: a database of quadruplex forming G-rich sequences in alternatively processed mammalian pre-mRNA sequences
Nucleic Acids Res., January 1, 2006; 34(suppl_1): D119 - D124.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
S. A. Shell, C. Hesse, S. M. Morris Jr., and C. Milcarek
Elevated Levels of the 64-kDa Cleavage Stimulatory Factor (CstF-64) in Lipopolysaccharide-stimulated Macrophages Influence Gene Expression and Induce Alternative Poly(A) Site Selection
J. Biol. Chem., December 2, 2005; 280(48): 39950 - 39961.
[Abstract] [Full Text] [PDF]

Home page
 RNAHome page
J. HU, C. S. LUTZ, J. WILUSZ, and B. TIAN
Bioinformatic identification of candidate cis-regulatory elements involved in human mRNA polyadenylation
RNA, October 1, 2005; 11(10): 1485 - 1493.
[Abstract] [Full Text] [PDF]

Home page
 J. Virol.Home page
D. Oberg, J. Fay, H. Lambkin, and S. Schwartz
A Downstream Polyadenylation Element in Human Papillomavirus Type 16 L2 Encodes Multiple GGG Motifs and Interacts with hnRNP H
J. Virol., July 15, 2005; 79(14): 9254 - 9269.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
D.-H. Kim, M.-A. Langlois, K.-B. Lee, A. D. Riggs, J. Puymirat, and J. J. Rossi
HnRNP H inhibits nuclear export of mRNA containing expanded CUG repeats and a distal branch point sequence
Nucleic Acids Res., July 15, 2005; 33(12): 3866 - 3874.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
B. Tian, J. Hu, H. Zhang, and C. S. Lutz
A large-scale analysis of mRNA polyadenylation of human and mouse genes
Nucleic Acids Res., January 12, 2005; 33(1): 201 - 212.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
E. Buratti, M. Baralle, L. De Conti, D. Baralle, M. Romano, Y. M. Ayala, and F. E. Baralle
hnRNP H binding at the 5' splice site correlates with the pathological effect of two intronic mutations in the NF-1 and TSH{beta} genes
Nucleic Acids Res., August 6, 2004; 32(14): 4224 - 4236.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
M. T. Vassileva and M. J. Matunis
SUMO Modification of Heterogeneous Nuclear Ribonucleoproteins
Mol. Cell. Biol., May 1, 2004; 24(9): 3623 - 3632.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
C. Wu and J. C. Alwine
Secondary Structure as a Functional Feature in the Downstream Region of Mammalian Polyadenylation Signals
Mol. Cell. Biol., April 1, 2004; 24(7): 2789 - 2796.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
M. I. Zarudnaya, I. M. Kolomiets, A. L. Potyahaylo, and D. M. Hovorun
Downstream elements of mammalian pre-mRNA polyadenylation signals: primary, secondary and higher-order structures
Nucleic Acids Res., March 1, 2003; 31(5): 1375 - 1386.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
B. J. Natalizio, L. C. Muniz, G. K. Arhin, J. Wilusz, and C. S. Lutz
Upstream Elements Present in the 3'-Untranslated Region of Collagen Genes Influence the Processing Efficiency of Overlapping Polyadenylation Signals
J. Biol. Chem., November 1, 2002; 277(45): 42733 - 42740.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
M. L. Peterson, S. Bertolino, and F. Davis
An RNA Polymerase Pause Site Is Associated with the Immunoglobulin {micro}s Poly(A) Site
Mol. Cell. Biol., August 1, 2002; 22(15): 5606 - 5615.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.