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Nucleic Acids Research, 2002, Vol. 30, No. 9 1935-1943
© 2002 Oxford University Press

Two self-splicing group I introns in the ribonucleotide reductase large subunit gene of Staphylococcus aureus phage Twort

Markus Landthaler, Ulrike Begley, Nelson C. Lau and David A. Shub*

Department of Biological Sciences and Center for Molecular Genetics, University at Albany, State University of New York, 1400 Washington Avenue, Albany, NY 12222, USA

We have recently described three group I introns inserted into a single gene, orf142, of the staphylococcal bacteriophage Twort and suggested the presence of at least two additional self-splicing introns in this phage genome. Here we report that two previously uncharacterized introns, 429 and 1087 nt in length, interrupt the Twort gene coding for the large subunit of ribonucleotide reductase (nrdE). Reverse transcription–polymerase chain reaction (RT–PCR) of RNA isolated from Staphylococcus aureus after phage infection indicates that the introns are removed from the primary transcript in vivo. Both nrdE introns show sequence similarity to the Twort orf142 introns I2 and I3, suggesting either a common origin of these introns or shuffling of intron structural elements. Intron 2 encodes a DNA endonuclease, I-TwoI, with similarity to homing endonucleases of the HNH family. Like I-HmuI and I-HmuII, intron-encoded HNH endonucleases in Bacillus subtilis phages SPO1 and SP82, I-TwoI nicks only one strand of its DNA recognition sequence. However, whereas I-HmuI and I-HmuII cleave the template strand in exon 2, I-TwoI cleaves the coding strand in exon 1. In each case, the 3' OH created on the cut strand is positioned to prime DNA synthesis towards the intron, suggesting that this reaction contributes to the mechanism of intron homing. Both nrdE introns are inserted in highly conserved regions of the ribonucleotide reductase gene, next to codons for functionally important residues.

* To whom correspondence should be addressed. Tel: +1 518 442 4324; Fax: +1 518 442 4354; Email: shub{at}albany.edu Present addresses:Ulrike Begley, Brigham and Women’s Hospital, Department of Medicine, Boston, MA 02115, USANelson C. Lau, Whitehead Institute for Biomedical Research, Massachusetts Institute of Technology, Cambridge, MA 02142, USA


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