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Nucleic Acids Research, 2002, Vol. 30, No. 9 2061-2067
© 2002 Oxford University Press

Human DNA polymerase mu (Pol µ) exhibits an unusual replication slippage ability at AAF lesion

Jean-Baptiste Duvauchelle, Luis Blanco1, Robert P. P. Fuchs and Agnes M. Cordonnier*

UPR9003 du CNRS, Cancérogenèse et Mutagenèse Moléculaire et Structurale, ESBS, Boulevard S. Brant, 67400 Strasbourg, France and 1Centro de Biología Molecular ‘Severo Ochoa’ (CSIC-UAM), Universidad Autónoma, 28049 Madrid, Spain

We analyzed the ability of various cell extracts to extend a radiolabeled primer past an N-2-acetylaminofluorene (AAF) adduct located on a primed single-stranded template. When the 3' end of the primer is located opposite the lesion, partially fractionated human primary fibroblast extracts efficiently catalyzed primer-terminus extension by adding a ladder of about 15 dGMPs, in an apparently non-templated reaction. This activity was not detected in SV40-transformed fibroblasts or in HeLa cell extracts unless purified human DNA polymerase mu (Pol µ) was added. In contrast, purified human Pol µ alone could only add three dGMPs as predicted from the sequence of the template. These results suggest that a cofactor(s) present in cellular extracts modifies Pol µ activity. The production of the dGMP ladder at the primer terminus located opposite the AAF adduct reveals an unusual ability of Pol µ (in conjunction with its cofactor) to perform DNA synthesis from a slipped intermediate containing several unpaired bases.

* To whom correspondence should be addressed. Tel: +33 390 24 4689; Fax: +33 390 24 4686; Email: agnes.cordonnier{at}esbs.u-strasbg.fr


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