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Nucleic Acids Research, 2002, Vol. 30, No. 9 2068-2075
© 2002 Oxford University Press

Functional characterization of the interferon regulatory element in the enhancer 1 region of the hepatitis B virus genome

Flavio F. Alcantara1, Hong Tang1,2 and Alan McLachlan1,*

1Department of Cell Biology, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA and 2Viral Hepatitis Research Unit, West China Hospital, West China Medical School, Sichuan University, Guo Xue Xiang, No. 37, Chengdu, Sichuan 610041, People’s Republic of China

An interferon-stimulated response element (ISRE)/interferon regulatory element (IRE) spanning nucleotide coordinates 1091–1100 is present in the enhancer 1/X gene promoter region of the hepatitis B virus (HBV) genome. In the context of a minimal promoter element, the enhancer 1/X gene promoter ISRE/IRE was shown to be a functional regulatory site capable of mediating interferon {alpha}- (IFN{alpha}) and interferon-stimulated gene factor 3 (ISGF3)-specific transcriptional activation in transient transfection analysis. The enhancer 1/X gene promoter ISRE/IRE was also shown to mediate interferon regulatory factor (IRF) 1 and IRF7 activation of transcription from a minimal promoter construct. In contrast, IFN{alpha} and the IRFs had minimal effect on HBV transcription and replication in the context of the viral genome in cell culture.

* To whom correspondence should be addressed. Tel: +1 858 784 8097; Fax: +1 858 784 2513; Email: mclach{at}scripps.edu


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