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Nucleic Acids Research, 2003, Vol. 31, No. 1 278-281
© 2003 Oxford University Press

BayGenomics: a resource of insertional mutations in mouse embryonic stem cells

Doug Stryke1, Michiko Kawamoto1, Conrad C. Huang1, Susan J. Johns1, Leslie A. King1, Courtney A. Harper1, Elaine C. Meng1, Roy E. Lee1, Alice Yee3, Larry L'Italien3, Pao-Tien Chuang2, Stephen G. Young3, William C. Skarnes4, Patricia C. Babbitt1 and Thomas E. Ferrin*,1

1 Departments of Pharmaceutical Chemistry and Biopharmaceutical Sciences, University of California San Francisco, 513 Parnassus Avenue, San Francisco, CA 94143, USA 2 Cardiovascular Research Institute, University of California San Francisco, 513 Parnassus Avenue, San Francisco, CA 94143, USA 3 Gladstone Institute of Cardiovascular Disease, 365 Vermont Street, San Francisco, CA 94103, USA 4 Department of Molecular and Cell Biology, University of California Berkeley, 589 Life Sciences Addition #3200, Berkeley, CA 94720, USA

*To whom correspondence should be addressed. Tel: +1 4154762299; Fax: +1 4155021755; Email: tef{at}cgl.ucsf.edu

ABSTRACT

The BayGenomics gene-trap resource (http://baygenomics.ucsf.edu) provides researchers with access to thousands of mouse embryonic stem (ES) cell lines harboring characterized insertional mutations in both known and novel genes. Each cell line contains an insertional mutation in a specific gene. The identity of the gene that has been interrupted can be determined from a DNA sequence tag. Approximately 75% of our cell lines contain insertional mutations in known mouse genes or genes that share strong sequence similarities with genes that have been identified in other organisms. These cell lines readily transmit the mutation to the germline of mice and many mutant lines of mice have already been generated from this resource. BayGenomics provides facile access to our entire database, including sequence tags for each mutant ES cell line, through the World Wide Web. Investigators can browse our resource, search for specific entries, download any portion of our database and BLAST sequences of interest against our entire set of cell line sequence tags. They can then obtain the mutant ES cell line for the purpose of generating knockout mice.


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