Nucleic Acids Research, 2003, Vol. 31, No. 1 393-396
© 2003 Oxford University Press
NESbase version 1.0: a database of nuclear export signals
1 Center for Biological Sequence Analysis, Building-208, Technical University of Denmark, DK-2800 Lyngby, Denmark 2 Department of Protein Chemistry, Institute of Molecular Biology, University of Copenhagen, DK-1353, Denmark
*To whom correspondence should be addressed. Tel: +45 45252477; Fax: +45 45931585; Email: brunak{at}cbs.dtu.dk
ABSTRACT
Protein export from the nucleus is often mediated by a Leucine-rich Nuclear Export Signal (NES). NESbase is a database of experimentally validated Leucine-rich NESs curated from literature. These signals are not annotated in databases such as SWISS-PROT, PIR or PROSITE. Each NESbase entry contains information of whether NES was shown to be necessary and/or sufficient for export, and whether the export was shown to be mediated by the export receptor CRM1. The compiled information was used to make a sequence logo of the Leucine-rich NESs, displaying the conservation of amino acids within a window of 25 residues. Surprisingly, only 36% of the sequences used for the logo fit the widely accepted NES consensus L-x(2,3)-[LIVFM]-x(2,3)-L-x-[LI]. The database is available online at http://www.cbs.dtu.dk/databases/NESbase/.
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