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Nucleic Acids Research, 2003, Vol. 31, No. 12 3157-3165
© 2003 Oxford University Press

The Upf-dependent decay of wild-type PPR1 mRNA depends on its 5'-UTR and first 92 ORF nucleotides

B. Kebaara, T. Nazarenus, R. Taylor, A. Forch and A. L. Atkin

School of Biological Sciences, University of Nebraska—Lincoln, Lincoln, NE 68588-0666, USA

*To whom correspondence should be addressed. Tel: +1 402 472 1411; Fax: +1 402 472 8722; Email: aatkin{at}biocomp.unl.edu

mRNAs containing premature translation termination codons (nonsense mRNAs) are targeted for deadenylation-independent degradation in a mechanism that depends on Upf1p, Upf2p and Upf3p. This decay pathway is often called nonsense- mediated mRNA decay (NMD). Nonsense mRNAs are decapped by Dcp1p and then degraded 5' to 3' by Xrn1p. In the yeast Saccharomyces cerevisiae, a significant number of wild-type mRNAs accumulate in upf mutants. Wild-type PPR1 mRNA is one of these mRNAs. Here we show that PPR1 mRNA degradation depends on the Upf proteins, Dcp1p, Xrn1p and Hrp1p. We have mapped an Upf1p-dependent destabilizing element to a region located within the 5'-UTR and the first 92 bases of the PPR1 ORF. This element targets PPR1 mRNA for Upf-dependent decay by a novel mechanism.


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