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Nucleic Acids Research, 2003, Vol. 31, No. 13 3406-3415
© 2003 Oxford University Press

Mfold web server for nucleic acid folding and hybridization prediction

Michael Zuker*

Department of Mathematical Sciences, Rensselaer Polytechnic Institute, Troy, NY 12180, USA

*Tel: +1 5182766902; Fax: +1 5182764824; Email: zukerm{at}rpi.edu

The abbreviated name, ‘mfold web server’, describes a number of closely related software applications available on the World Wide Web (WWW) for the prediction of the secondary structure of single stranded nucleic acids. The objective of this web server is to provide easy access to RNA and DNA folding and hybridization software to the scientific community at large. By making use of universally available web GUIs (Graphical User Interfaces), the server circumvents the problem of portability of this software. Detailed output, in the form of structure plots with or without reliability information, single strand frequency plots and ‘energy dot plots’, are available for the folding of single sequences. A variety of ‘bulk’ servers give less information, but in a shorter time and for up to hundreds of sequences at once. The portal for the mfold web server is http://www.bioinfo.rpi.edu/applications/mfold. This URL will be referred to as ‘MFOLDROOT’.


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B. P. Higgins, A. C. Popkowski, P. R. Caruana, and A. C. Karls
Site-Specific Insertion of IS492 in Pseudoalteromonas atlantica
J. Bacteriol., October 15, 2009; 191(20): 6408 - 6414.
[Abstract] [Full Text] [PDF]


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J. Bacteriol.Home page
G. Leon and P. H. Roy
Potential Role of Group IIC-attC Introns in Integron Cassette Formation
J. Bacteriol., October 1, 2009; 191(19): 6040 - 6051.
[Abstract] [Full Text] [PDF]


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RNAHome page
S. Liang and G. J. Connell
An electrochemiluminescent aptamer switch for a high-throughput assay of an RNA editing reaction
RNA, October 1, 2009; 15(10): 1929 - 1938.
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Nucleic Acids ResHome page
Y. Kino, C. Washizu, Y. Oma, H. Onishi, Y. Nezu, N. Sasagawa, N. Nukina, and S. Ishiura
MBNL and CELF proteins regulate alternative splicing of the skeletal muscle chloride channel CLCN1
Nucleic Acids Res., October 1, 2009; 37(19): 6477 - 6490.
[Abstract] [Full Text] [PDF]


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Nucleic Acids ResHome page
M. L. Yeung, Y. Bennasser, K. Watashi, S.-Y. Le, L. Houzet, and K.-T. Jeang
Pyrosequencing of small non-coding RNAs in HIV-1 infected cells: evidence for the processing of a viral-cellular double-stranded RNA hybrid
Nucleic Acids Res., October 1, 2009; 37(19): 6575 - 6586.
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Syst BiolHome page
L. Leclere, P. Schuchert, C. Cruaud, A. Couloux, and M. Manuel
Molecular Phylogenetics of Thecata (Hydrozoa, Cnidaria) Reveals Long-Term Maintenance of Life History Traits despite High Frequency of Recent Character Changes
Syst Biol, October 1, 2009; 58(5): 509 - 526.
[Abstract] [Full Text] [PDF]


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J. Biol. Chem.Home page
G. Deikus and D. H. Bechhofer
Bacillus subtilis trp Leader RNA: RNase J1 ENDONUCLEASE CLEAVAGE SPECIFICITY AND PNPase PROCESSING
J. Biol. Chem., September 25, 2009; 284(39): 26394 - 26401.
[Abstract] [Full Text] [PDF]


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J. Biol. Chem.Home page
J. A. Mertz, A. B. Chadee, H. Byun, R. Russell, and J. P. Dudley
Mapping of the Functional Boundaries and Secondary Structure of the Mouse Mammary Tumor Virus Rem-responsive Element
J. Biol. Chem., September 18, 2009; 284(38): 25642 - 25652.
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Proc. Natl. Acad. Sci. USAHome page
J. M. Peters, R. A. Mooney, P. F. Kuan, J. L. Rowland, S. Keles, and R. Landick
Rho directs widespread termination of intragenic and stable RNA transcription
PNAS, September 8, 2009; 106(36): 15406 - 15411.
[Abstract] [Full Text] [PDF]


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Acta Biochim Biophys SinHome page
Y. Zhang and G. Hong
Post-transcriptional regulation of NifA expression by Hfq and RNase E complex in Rhizobium leguminosarum bv. viciae
Acta Biochim Biophys Sin, September 1, 2009; 41(9): 719 - 730.
[Abstract] [Full Text] [PDF]


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Nucleic Acids ResHome page
R. S. P. Horler and C. K. Vanderpool
Homologs of the small RNA SgrS are broadly distributed in enteric bacteria but have diverged in size and sequence
Nucleic Acids Res., September 1, 2009; 37(16): 5465 - 5476.
[Abstract] [Full Text] [PDF]


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J. Bacteriol.Home page
Y. Terui, K. Higashi, Y. Tabei, H. Tomitori, K. Yamamoto, A. Ishihama, K. Igarashi, and K. Kashiwagi
Enhancement of the Synthesis of RpoE and StpA by Polyamines at the Level of Translation in Escherichia coli under Heat Shock Conditions
J. Bacteriol., September 1, 2009; 191(17): 5348 - 5357.
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Nucleic Acids ResHome page
S. Chirayil, R. Chirayil, and K. J. Luebke
Discovering ligands for a microRNA precursor with peptoid microarrays
Nucleic Acids Res., September 1, 2009; 37(16): 5486 - 5497.
[Abstract] [Full Text] [PDF]



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