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Nucleic Acids Research, 2003, Vol. 31, No. 13 3859-3861
© 2003 Oxford University Press

CRP: Cleavage of Radiolabeled Phosphoproteins

Aaron J. Mackey, Timothy A.J. Haystead2 and William R. Pearson*,1

Department of Microbiology, University of Virginia, Charlottesville, VA 22908, USA 1 Department of Biochemistry and Molecular Genetics, University of Virginia, Charlottesville, VA 22908, USA 2 Department of Pharmacology and Cancer Biology, Duke University, Durham, NC 27710, USA

*To whom correspondence should be addressed. Tel: +1 4349242818; Fax: +1 4349245069; Email: wrp{at}virginia.edu

The CRP (Cleavage of Radiolabeled Phosphoproteins) program guides the design and interpretation of experiments to identify protein phosphorylation sites by Edman sequencing of unseparated peptides. Traditionally, phosphorylation sites are determined by cleaving the phosphoprotein and separating the peptides for Edman 32P-phosphate release sequencing. CRP analysis of a phosphoprotein's sequence accelerates this process by omitting the separation step: given a protein sequence of interest, the CRP program performs an in silico proteolytic cleavage of the sequence and reports the predicted Edman cycles in which radioactivity would be observed if a given serine, threonine or tyrosine were phosphorylated. Experimentally observed cycles containing 32P can be compared with CRP predictions to confirm candidate sites and/or explore the ability of additional cleavage experiments to resolve remaining ambiguities. To reduce ambiguity, the phosphorylated residue (P-Tyr, P-Ser or P-Thr) can be determined experimentally, and CRP will ignore sites with alternative residues. CRP also provides simple predictions of likely phosphorylation sites using known kinase recognition motifs. The CRP interface is available at http://fasta.bioch.virginia.edu/crp.


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