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Nucleic Acids Research, 2003, Vol. 31, No. 14 4201-4210
© 2003 Oxford University Press

Leishmania major chromosome 3 contains two long convergent polycistronic gene clusters separated by a tRNA gene

E. A. Worthey1, Santiago Martinez-Calvillo1, Achim Schnaufer1, Gautam Aggarwal1, Jason Cawthra1, Gholam Fazelinia1, Chris Fong1, Guoliang Fu2, Melissa Hassebrock1, Greg Hixson1, Alasdair C. Ivens3, Patti Kiser1, Felicia Marsolini1, Erica Rickell1, Reza Salavati1, Ellen Sisk1, Susan M. Sunkin1, Kenneth D. Stuart1,4 and Peter J. Myler*,1,4,5

1 Seattle Biomedical Research Institute, 4 Nickerson Street, Seattle, WA 98109-1651, USA, 2 Department of Pathology, University of Cambridge, Cambridge CB2 1QP, UK, 3 Wellcome Trust Sanger Institute, Hinxton CB10 1SA, UK, 4 Department of Pathobiology, University of Washington, Seattle, WA 98195, USA and 5 Department of Medical Education and Biomedical Informatics, University of Washington, Seattle, WA 98195, USA

*To whom correspondence should be addressed at Seattle Biomedical Research Institute, 4 Nickerson Street, Seattle, WA 98109-1651, USA. Tel: +1 206 284 8846; Fax: +1 206 284 0313; Email: peter.myler{at}sbri.org

Leishmania parasites (order Kinetoplastida, family Trypanosomatidae) cause a spectrum of human diseases ranging from asymptomatic to lethal. The ~33.6 Mb genome is distributed among 36 chromosome pairs that range in size from ~0.3 to 2.8 Mb. The complete nucleotide sequence of Leishmania major Friedlin chromosome 1 revealed 79 protein-coding genes organized into two divergent polycistronic gene clusters with the mRNAs transcribed towards the telomeres. We report here the complete nucleotide sequence of chromosome 3 (384 518 bp) and an analysis revealing 95 putative protein-coding ORFs. The ORFs are primarily organized into two large convergent polycistronic gene clusters (i.e. transcribed from the telomeres). In addition, a single gene at the left end is transcribed divergently towards the telomere, and a tRNA gene separates the two convergent gene clusters. Numerous genes have been identified, including those for metabolic enzymes, kinases, transporters, ribosomal proteins, spliceosome components, helicases, an RNA-binding protein and a DNA primase subunit.


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