Nucleic Acids Research, 2003, Vol. 31, No. 14 4256-4263
© 2003 Oxford University Press
Formation of stable triplexes between purine RNA and pyrimidine oligodeoxyxylonucleotides
1 Belozersky Institute of Physico-Chemical Biology and Department of Chemistry, Moscow State University, Moscow 119899, Russia, 2 Syntol Corporation, Timiryazevskaya Street 42, Moscow 127550, Russia and 3 CNRS UMR 8121, Institut Gustave Roussy, Rue Camille Desmoulins, 94805 Villejuif Cedex, France
*To whom correspondence should be addressed. Tel: +33 142 11 5045; Fax: +33 142 11 5245; Email: jrb{at}igr.fr
This article is dedicated to the memory of our colleague, Prof. Claude Helene, who died on February 11, 2003. He was known nationally and internationally for his work on nucleic acids and more particularly on triple helices. He was always open to discussions with and advice to his colleagues.
Hybridization properties of oligodeoxyxylonucleotides (OXNs) built from pyrimidine monomers with an inverted 3'-OH group of the furanose have been studied using the gel mobility shift, UV melting and circular dichroism (CD) spectroscopy methods. Pyrimidine OXNs form triple helices with complementary purine RNA in which one OXN is parallel and another is antiparallel with respect to the RNA target. Surprisingly, no duplex formation between the pyrimidine OXNs and purine RNAs is detected. The modified triplexes are stable at pH 7. Their thermal stability depends on the number of C(G-C) triplets and, for G-rich RNA sequences, it is comparable with the stability of native DNA–RNA duplexes. The CD spectra of triplexes formed by OXNs with purine RNA targets are similar to spectra of A-type helices. A pyrimidine OXN having a clamp structure efficiently inhibits reverse transcription of murine pim-1 mRNA in vitro mediated by the Mo-MuLV reverse transcriptase.
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