Nucleic Acids Research, 2003, Vol. 31, No. 15 4361-4372
© 2003 Oxford University Press
Mutagenesis of a light-regulated psbA intron reveals the importance of efficient splicing for photosynthetic growth
Molecular Cell and Developmental Biology Section and Institute for Cellular and Molecular Biology, School of Biological Sciences, 1 University Station A6700, University of Texas at Austin, Austin, TX 78712, USA
*To whom correspondence should be addressed. Tel: +1 512 471 3843; Fax: +1 512 471 3843; Email: dlherrin{at}utxvms.cc.utexas.edu
The chloroplast-encoded psbA gene encodes the D1 polypeptide of the photosystem II reaction center, which is synthesized at high rates in the light. In Chlamydomonas reinhardtii, the psbA gene contains four self-splicing group I introns whose rates of splicing in vivo are increased at least 6–10-fold by light. However, because psbA is an abundant mRNA, and some chloroplast mRNAs appear to be in great excess of what is needed to sustain translation rates, the developmental significance of light-promoted splicing has not been clear. To address this and other questions, potentially destabilizing substitutions were made in several predicted helices of the fourth psbA intron, Cr.psbA4, and their effects on in vitro and in vivo splicing assessed. Two-nucleotide substitutions in P4 and P7 were necessary to substantially reduce splicing of this intron in vivo, although most mutations reduced self-splicing in vitro. The P7-4,5 mutant, whose splicing was completely blocked, showed no photoautotrophic growth and synthesis of a truncated D1 (exons 1–4) polypeptide from the unspliced mRNA. Most informative was the P4'-3,4 mutant, which exhibited a 45% reduction in spliced psbA mRNA, a 28% reduction in synthesis of full-length D1, and an 18% reduction in photoautotrophic growth. These results indicate that psbA mRNA is not in great excess, and that highly efficient splicing of psbA introns, which is afforded by light conditions, is necessary for optimal photosynthetic growth.
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  J. Uniacke and W. Zerges Photosystem II Assembly and Repair Are Differentially Localized in Chlamydomonas PLANT CELL, November 1, 2007; 19(11): 3640 - 3654. [Abstract] [Full Text] [PDF]
|
|