Skip Navigation

This Article
Right arrow Full Text Freely available
Right arrow Print PDF (201K) Freely available
Right arrow Supplementary Material
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (40)
Right arrowRequest Permissions
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Buzdin, A.
Right arrow Articles by Sverdlov, E.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Buzdin, A.
Right arrow Articles by Sverdlov, E.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Nucleic Acids Research, 2003, Vol. 31, No. 15 4385-4390
© 2003 Oxford University Press

The human genome contains many types of chimeric retrogenes generated through in vivo RNA recombination

Anton Buzdin*, Elena Gogvadze, Elena Kovalskaya, Pavel Volchkov, Svetlana Ustyugova, Anna Illarionova, Alexey Fushan, Tatiana Vinogradova and Eugene Sverdlov

Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Moscow 117871, Russia

*To whom correspondence should be addressed. Tel: +7 095 3306329; Fax: +7 095 3306538; Email: anton{at}humgen.siobc.ras.ru

L1 retrotransposons play an important role in mammalian genome shaping. In particular, they can transduce their 3'-flanking regions to new genomic loci or produce pseudogenes or retrotranscripts through reverse transcription of different kinds of cellular RNAs. Recently, we found in the human genome an unusual family of chimeric retrotranscripts composed of full-sized copies of U6 small nuclear RNAs fused at their 3' termini with 5'-truncated, 3'-poly(A)-tailed L1s. The chimeras were flanked by 11–21 bp long direct repeats, and contained near their 5' ends T2A4 hexanucleotide motifs, preferably recognized by L1 nicking endonuclease. These features suggest that the chimeras were formed using the L1 integration machinery. Here we report the identification of 81 chimeras consisting of fused DNA copies of different RNAs, including mRNAs of known human genes. Based on their structural features, the chimeras were subdivided into nine distinct families. 5' Parts of the chimeras usually originated from different nuclear RNAs, whereas their 3' parts represented cytoplasmic RNAs: mRNAs, including L1 mRNA and Alu RNA. Some of these chimeric retrotranscripts are expressed in a variety of human tissues. These findings suggest that RNA–RNA recombination during L1 reverse transcription followed by the integration of the recombinants into the host genome is a general event in genome evolution.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Mol. Cell. Biol.Home page
J. L. Goodier, L. Zhang, M. R. Vetter, and H. H. Kazazian Jr.
LINE-1 ORF1 Protein Localizes in Stress Granules with Other RNA-Binding Proteins, Including Components of RNA Interference RNA-Induced Silencing Complex
Mol. Cell. Biol., September 15, 2007; 27(18): 6469 - 6483.
[Abstract] [Full Text] [PDF]

Home page
 Hum Mol GenetHome page
J. L. Garcia-Perez, M. C.N. Marchetto, A. R. Muotri, N. G. Coufal, F. H. Gage, K. S. O'Shea, and J. V. Moran
LINE-1 retrotransposition in human embryonic stem cells
Hum. Mol. Genet., July 1, 2007; 16(13): 1569 - 1577.
[Abstract] [Full Text] [PDF]

Home page
 Genome ResHome page
J. L. Garcia-Perez, A. J. Doucet, A. Bucheton, J. V. Moran, and N. Gilbert
Distinct mechanisms for trans-mediated mobilization of cellular RNAs by the LINE-1 reverse transcriptase
Genome Res., May 1, 2007; 17(5): 602 - 611.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
Y. L. a. S. Li
Genome-wide analyses of retrogenes derived from the human box H/ACA snoRNAs
Nucleic Acids Res., January 28, 2007; 35(2): 559 - 571.
[Abstract] [Full Text] [PDF]

Home page
 Syst BiolHome page
D. Kordis, N. Lovsin, and F. Gubensek
Phylogenomic Analysis of the L1 Retrotransposons in Deuterostomia
Syst Biol, December 1, 2006; 55(6): 886 - 901.
[Abstract] [Full Text] [PDF]

Home page
 J. Virol.Home page
A. Buzdin, E. Kovalskaya-Alexandrova, E. Gogvadze, and E. Sverdlov
At Least 50% of Human-Specific HERV-K (HML-2) Long Terminal Repeats Serve In Vivo as Active Promoters for Host Nonrepetitive DNA Transcription.
J. Virol., November 1, 2006; 80(21): 10752 - 10762.
[Abstract] [Full Text] [PDF]

Home page
 Genome ResHome page
H. Nishihara, A. F.A. Smit, and N. Okada
Functional noncoding sequences derived from SINEs in the mammalian genome
Genome Res., July 1, 2006; 16(7): 864 - 874.
[Abstract] [Full Text] [PDF]

Home page
 Genome ResHome page
D. V. Babushok, E. M. Ostertag, C. E. Courtney, J. M. Choi, and H. H. Kazazian Jr.
L1 integration in a transgenic mouse model
Genome Res., February 1, 2006; 16(2): 240 - 250.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
N. Gilbert, S. Lutz, T. A. Morrish, and J. V. Moran
Multiple Fates of L1 Retrotransposition Intermediates in Cultured Human Cells
Mol. Cell. Biol., September 1, 2005; 25(17): 7780 - 7795.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
J. Perreault, J.-F. Noel, F. Briere, B. Cousineau, J.-F. Lucier, J.-P. Perreault, and G. Boire
Retropseudogenes derived from the human Ro/SS-A autoantigen-associated hY RNAs
Nucleic Acids Res., April 7, 2005; 33(6): 2032 - 2041.
[Abstract] [Full Text] [PDF]

Home page
 Genome ResHome page
S. Boissinot, A. Entezam, L. Young, P. J. Munson, and A. V. Furano
The Insertional History of an Active Family of L1 Retrotransposons in Humans
Genome Res., July 1, 2004; 14(7): 1221 - 1231.
[Abstract] [Full Text] [PDF]

Home page
 Hum Mol GenetHome page
J. L. Goodier, E. M. Ostertag, K. A. Engleka, M. C. Seleme, and H. H. Kazazian Jr
A potential role for the nucleolus in L1 retrotransposition
Hum. Mol. Genet., May 15, 2004; 13(10): 1041 - 1048.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
A. Bibillo and T. H. Eickbush
End-to-End Template Jumping by the Reverse Transcriptase Encoded by the R2 Retrotransposon
J. Biol. Chem., April 9, 2004; 279(15): 14945 - 14953.
[Abstract] [Full Text] [PDF]

Home page
 ScienceHome page
H. H. Kazazian Jr.
Mobile Elements: Drivers of Genome Evolution
Science, March 12, 2004; 303(5664): 1626 - 1632.
[Abstract] [Full Text] [PDF]

Home page
 RNAHome page
K. E. GILES, M. CAPUTI, and K. L. BEEMON
Packaging and reverse transcription of snRNAs by retroviruses may generate pseudogenes
RNA, February 1, 2004; 10(2): 299 - 307.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.