Skip Navigation

This Article
Right arrow Full Text Freely available
Right arrow Print PDF (199K) Freely available
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (19)
Right arrowRequest Permissions
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by D’souza, D. I.
Right arrow Articles by Harrison, L.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by D’souza, D. I.
Right arrow Articles by Harrison, L.
Related Collections
Right arrow Repair
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Nucleic Acids Research, 2003, Vol. 31, No. 15 4573-4581
© 2003 Oxford University Press

Repair of clustered uracil DNA damages in Escherichia coli

Dwain I. D’souza and Lynn Harrison*

Department of Molecular and Cellular Physiology, Louisiana Health Sciences Center, Shreveport, LA, USA

*To whom correspondence should be addressed. Tel: +1 318 675 4213; Fax: +1 318 675 4217; Email: lclary{at}lsuhsc.edu

Multiply damaged sites (MDS) are defined as greater than/equal to two lesions within 10–15 bp and are generated in DNA by ionizing radiation. In vitro repair of closely opposed base damages >=2 bp apart results in a double strand break (DSB). This work extends the in vitro studies by utilizing clusters of uracil DNA damage as model lesions to determine whether MDS are converted to DSBs in bacteria. Lesions were positioned within the firefly luciferase coding region, transformed into bacteria (wild-type, uracil DNA glycosylase-deficient, ung–, or exonuclease III and endonuclease IV-deficient, xth–nfo–) and luciferase activity measured following repair. DSB formation was expected to decrease activity. Two closely opposed uracils separated by <=7 bp decreased luciferase activity in wild-type and xth–nfo–, but not ung– bacteria. Growth of bacteria to obtain plasmid-containing colonies demonstrated that the plasmid was destroyed following the mis-repair of two uracils positioned 7 bp apart. This study indicates a DSB is formed when uracil DNA glycosylase initiates repair of two closely opposed uracils <=7 bp apart, even in the absence of the major apurinic endonucleases. This work supports the in vitro studies and demonstrates that DNA repair is not always advantageous to cells.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Nucleic Acids ResHome page
S. Malyarchuk, R. Castore, and L. Harrison
DNA repair of clustered lesions in mammalian cells: involvement of non-homologous end-joining
Nucleic Acids Res., September 1, 2008; 36(15): 4872 - 4882.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
B. Paap, D. M. Wilson III, and B. M. Sutherland
Human abasic endonuclease action on multilesion abasic clusters: implications for radiation-induced biological damage
Nucleic Acids Res., May 1, 2008; 36(8): 2717 - 2727.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
S. Mourgues, M. E. Lomax, and P. O'Neill
Base excision repair processing of abasic site/single-strand break lesions within clustered damage sites associated with XRCC1 deficiency
Nucleic Acids Res., December 3, 2007; 35(22): 7676 - 7687.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
G. Eot-Houllier, M. Gonera, D. Gasparutto, C. Giustranti, and E. Sage
Interplay between DNA N-glycosylases/AP lyases at multiply damaged sites and biological consequences
Nucleic Acids Res., May 11, 2007; 35(10): 3355 - 3366.
[Abstract] [Full Text] [PDF]

Home page
 Radiat Prot DosimetryHome page
S. Shchemelinin, G. Hilgers, E. Gargioni, B. Grosswendt, A. Breskin, and R. Chechik
Dependence of nanodosimetric spectra on the sensitive volume length and ion drift in an ion-counting nanodosemeter
Radiat Prot Dosimetry, December 1, 2006; 122(1-4): 446 - 450.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
N. Shikazono, C. Pearson, P. O'Neill, and J. Thacker
The roles of specific glycosylases in determining the mutagenic consequences of clustered DNA base damage
Nucleic Acids Res., August 7, 2006; 34(13): 3722 - 3730.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
G.ég. Eot-Houllier, Sév. Eon-Marchais, D. Gasparutto, and E. Sage
Processing of a complex multiply damaged DNA site by human cell extracts and purified repair proteins
Nucleic Acids Res., January 12, 2005; 33(1): 260 - 271.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
S. Malyarchuk, K. L. Brame, R. Youngblood, R. Shi, and L. Harrison
Two clustered 8-oxo-7,8-dihydroguanine (8-oxodG) lesions increase the point mutation frequency of 8-oxodG, but do not result in double strand breaks or deletions in Escherichia coli
Nucleic Acids Res., October 27, 2004; 32(19): 5721 - 5731.
[Abstract] [Full Text] [PDF]

Home page
 GeneticsHome page
C. Saint-Ruf, F. Taddei, and I. Matic
Stress and Survival of Aging Escherichia coli rpoS Colonies
Genetics, September 1, 2004; 168(1): 541 - 546.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
M. Gulston, C. de Lara, T. Jenner, E. Davis, and P. O'Neill
Processing of clustered DNA damage generates additional double-strand breaks in mammalian cells post-irradiation
Nucleic Acids Res., March 5, 2004; 32(4): 1602 - 1609.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
C. G. Pearson, N. Shikazono, J. Thacker, and P. O'Neill
Enhanced mutagenic potential of 8-oxo-7,8-dihydroguanine when present within a clustered DNA damage site
Nucleic Acids Res., January 9, 2004; 32(1): 263 - 270.
[Abstract] [Full Text] [PDF]


Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.