Skip Navigation

This Article
Right arrow Full Text Freely available
Right arrow Print PDF (127K) Freely available
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Brown, A. E.
Right arrow Articles by Catteruccia, F.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Brown, A. E.
Right arrow Articles by Catteruccia, F.
Related Collections
Right arrow Targeted inhibition of gene function
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Nucleic Acids Research, 2003, Vol. 31, No. 15 e85
© 2003 Oxford University Press

Stable and heritable gene silencing in the malaria vector Anopheles stephensi

Anthony E. Brown1, Laurence Bugeon1,2, Andrea Crisanti1 and Flaminia Catteruccia*,1

1 Department of Biological Sciences and 2 CMMI, SAF Building, Imperial College London, Imperial College Road, London SW7 2AZ, UK

*To whom correspondence should be addressed. Tel: +44 20 75945412; Fax: +44 20 75945439; Email: f.catteruccia{at}imperial.ac.uk

Heritable RNA interference (RNAi), triggered from stably expressed transgenes with an inverted repeat (IR) configuration, is an important tool for reverse genetic studies. Here we report on the development of stable RNAi in Anopheles stephensi mosquitoes, the major vector of human malaria in Asia. Trans genic mosquitoes stably expressing a RNAi transgene, designed to produce intron-spliced double-stranded RNA (dsRNA) targeting the green fluorescent protein EGFP gene, were crossed to an EGFP-expressing target line. EGFP expression was dramatically reduced at both the protein and RNA levels. The levels of gene silencing depended upon the RNAi gene copy number and its site of integration. These results demonstrate that specific RNAi-mediated knockdown of gene function can be achieved with high efficiency in Anopheles. This will be invaluable to systematically unravel the function of Anopheles genes determining the vectorial capacity of the malaria parasite.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Nucleic Acids ResHome page
V. De Riso, R. Raniello, F. Maumus, A. Rogato, C. Bowler, and A. Falciatore
Gene silencing in the marine diatom Phaeodactylum tricornutum
Nucleic Acids Res., May 31, 2009; (2009) gkp448v1.
[Abstract] [Full Text] [PDF]

Home page
 GeneticsHome page
G. J. Lycett, F. C. Kafatos, and T. G. Loukeris
Conditional Expression in the Malaria Mosquito Anopheles stephensi With Tet-On and Tet-Off Systems
Genetics, August 1, 2004; 167(4): 1781 - 1790.
[Abstract] [Full Text] [PDF]

Home page
 J. Exp. Biol.Home page
M. A. Osta, G. K. Christophides, D. Vlachou, and F. C. Kafatos
Innate immunity in the malaria vector Anopheles gambiae: comparative and functional genomics
J. Exp. Biol., July 1, 2004; 207(15): 2551 - 2563.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.