Skip Navigation

This Article
Right arrow Full Text Freely available
Right arrow Print PDF (311K) Freely available
Right arrow Supplementary Material
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (28)
Right arrowRequest Permissions
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Boutla, A.
Right arrow Articles by Tabler, M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Boutla, A.
Right arrow Articles by Tabler, M.
Related Collections
Right arrow Targeted inhibition of gene function
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Nucleic Acids Research, 2003, Vol. 31, No. 17 4973-4980
© 2003 Oxford University Press

Developmental defects by antisense-mediated inactivation of micro-RNAs 2 and 13 in Drosophila and the identification of putative target genes

Alexandra Boutla1,2, Christos Delidakis1,2 and Martin Tabler*,1

1 Institute of Molecular Biology and Biotechnology, Foundation for Research and Technology, Hellas, PO Box 1527, GR-71110 Heraklion/Crete, Greece and 2 Department of Biology, University of Crete, GR-71110 Heraklion/Crete, Greece

*To whom correspondence should be addressed. Tel: +30 2810 394 365; Fax: +30 2810 394 408; Email: tabler{at}imbb.forth.gr

Micro-RNAs are a class of small non-coding regulatory RNAs that impair translation by imperfect base pairing to mRNAs. For analysis of their cellular function we injected different miRNA-specific DNA antisense oligonucleotides in Drosophila embryos. In four cases we observed severe interference with normal development, one had a moderate impact and six oligonucleotides did not cause detectable phenotypes. We further used the miR-13a DNA antisense oligonucleotide as a PCR primer on a cDNA library template. In this experimental way we identified nine Drosophila genes, which are characterised by 3' untranslated region motifs that allow imperfect duplex formation with miR-13 or related miRNAs. These genes, which include Sos and Myd88, represent putative targets for miRNA regulation. Mutagenesis of the target motif of two genes followed by transfection in Drosophila Schneider 2 (S2) cells and subsequent reporter gene analysis confirmed the hypothesis that the binding potential of miR-13 is inversely correlated with gene expression.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 RNAHome page
V. Vopalensky, T. Masek, O. Horvath, B. Vicenova, M. Mokrejs, and M. Pospisek
Firefly luciferase gene contains a cryptic promoter
RNA, September 1, 2008; 14(9): 1720 - 1729.
[Abstract] [Full Text] [PDF]

Home page
 Clin. Cancer Res.Home page
A. J. Lowery, N. Miller, R. E. McNeill, and M. J. Kerin
MicroRNAs as Prognostic Indicators and Therapeutic Targets: Potential Effect on Breast Cancer Management
Clin. Cancer Res., January 15, 2008; 14(2): 360 - 365.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
S. Davis, B. Lollo, S. Freier, and C. Esau
Improved targeting of miRNA with antisense oligonucleotides.
Nucleic Acids Res., January 1, 2006; 34(8): 2294 - 2304.
[Abstract] [Full Text] [PDF]

Home page
 RNAHome page
O. SAeTROM, O. SNOVE JR., and P. SAeTROM
Weighted sequence motifs as an improved seeding step in microRNA target prediction algorithms
RNA, July 1, 2005; 11(7): 995 - 1003.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
V. Rusinov, V. Baev, I. N. Minkov, and M. Tabler
MicroInspector: a web tool for detection of miRNA binding sites in an RNA sequence
Nucleic Acids Res., July 1, 2005; 33(suppl_2): W696 - W700.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
A. M. Cheng, M. W. Byrom, J. Shelton, and L. P. Ford
Antisense inhibition of human miRNAs and indications for an involvement of miRNA in cell growth and apoptosis
Nucleic Acids Res., March 1, 2005; 33(4): 1290 - 1297.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
C. Esau, X. Kang, E. Peralta, E. Hanson, E. G. Marcusson, L. V. Ravichandran, Y. Sun, S. Koo, R. J. Perera, R. Jain, et al.
MicroRNA-143 Regulates Adipocyte Differentiation
J. Biol. Chem., December 10, 2004; 279(50): 52361 - 52365.
[Abstract] [Full Text] [PDF]

Home page
 RNAHome page
G. MEISTER, M. LANDTHALER, Y. DORSETT, and T. TUSCHL
Sequence-specific inhibition of microRNA- and siRNA-induced RNA silencing
RNA, March 1, 2004; 10(3): 544 - 550.
[Abstract] [Full Text] [PDF]

Home page
 Genes Dev.Home page
J. G. Doench and P. A. Sharp
Specificity of microRNA target selection in translational repression
Genes & Dev., March 1, 2004; 18(5): 504 - 511.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.