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Nucleic Acids Research, 2003, Vol. 31, No. 18 5256-5265
© 2003 Oxford University Press

Light-mediated regulation defines a minimal promoter region of TOP2

G. H. C. M. Hettiarachchi1,2, Vandana Yadav1, M. K. Reddy2, Sudip Chattopadhyay*,1 and Sudhir K. Sopory2

1 National Center for Plant Genome Research, PO Box 10531, JNU Campus, New Delhi 110067, India and 2 International Center for Genetic Engineering and Biotechnology, Aruna Asaf Ali Marg, New Delhi 110067, India

*To whom correspondence should be addressed. Tel: +91 11 2670 4531; Fax: +91 11 2616 7394; Email: sudipchatto{at}yahoo.com

Light signaling has been demonstrated to be an important factor for plant growth and development; however, its role in the regulation of DNA replication and cell cycle has just started to be unraveled. In this work, we have demonstrated that the TOP2 promoter of Pisum sativum (pea) is activated by a broad spectrum of light including far-red light (FR), red light (RL) and blue light (BL). Deletion analyses of the TOP2 promoter in transformed plants, Arabidopsis thaliana and Nicotiana tobaccum (tobacco), define a minimal promoter region that is induced by RL, FR and BL, and is essential and sufficient for light-mediated activation. The minimal promoter of TOP2 follows the phytochrome- mediated low-fluence response similar to complex light regulated promoters. DNA–protein interaction studies reveal the presence of a DNA binding activity specific to a 106 bp region of the minimal promoter that is crucial for light-mediated activation. These results altogether indicate a direct involvement of light signaling in the regulation of expression of TOP2, one of the components of the DNA replication/cell cycle machinery.


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