Skip Navigation

This Article
Right arrow Full Text Freely available
Right arrow Print PDF (188K) Freely available
Right arrow Supplementary Material
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (16)
Right arrowRequest Permissions
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Cesnaviciene, E.
Right arrow Articles by Lubys, A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Cesnaviciene, E.
Right arrow Articles by Lubys, A.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Nucleic Acids Research, 2003, Vol. 31, No. 2 743-749
© 2003 Oxford University Press

Esp1396I restriction–modification system: structural organization and mode of regulation

Egle Cesnaviciene, Goda Mitkaite1, Kornelijus Stankevicius, Arvydas Janulaitis and Arvydas Lubys*

Institute of Biotechnology and 1 Fermentas UAB, Graiciuno 8, 2028 Vilnius, Lithuania

*To whom correspondence should be addressed. Tel: +370 2 602105; Fax: +370 2 602116; Email: lubys{at}ibt.lt
Present address:
Egle Cesnaviciene, Fermentas UAB, Graiciuno 8, 2028 Vilnius, Lithuania

Esp1396I restriction–modification (RM) system recognizes an interrupted palindromic DNA sequ ence 5'-CCA(N)5TGG-3'. The Esp1396I RM system was found to reside on pEsp1396, a 5.6 kb plasmid naturally occurring in Enterobacter sp. strain RFL1396. The nucleotide sequence of the entire 5622 bp pEsp1396 plasmid was determined on both strands. Identified genes for DNA methyltransferase (esp1396IM) and restriction endonuclease (esp1396IR) are transcribed convergently. The restriction endonuclease gene is preceded by the small ORF (esp1396IC) that possesses a strong helix-turn-helix motif and resembles regulatory proteins found in PvuII, BamHI and few other RM systems. Gene regulation studies revealed that C.Esp1396I acts as both a repressor of methylase expression and an activator of regulatory protein and restriction endonuclease expression. Our data indicate that C protein from Esp1396I RM system activates the expression of the Enase gene, which is co-transcribed from the promoter of regulatory gene, by the mechanism of coupled translation.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Nucleic Acids ResHome page
E. Bogdanova, M. Zakharova, S. Streeter, J. Taylor, T. Heyduk, G. Kneale, and K. Severinov
Transcription regulation of restriction-modification system Esp1396I
Nucleic Acids Res., June 1, 2009; 37(10): 3354 - 3366.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
V. Sorokin, K. Severinov, and M. S. Gelfand
Systematic prediction of control proteins and their DNA binding sites
Nucleic Acids Res., February 1, 2009; 37(2): 441 - 451.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
I. Mruk and R. M. Blumenthal
Tuning the relative affinities for activating and repressing operators of a temporally regulated restriction-modification system
Nucleic Acids Res., February 1, 2009; 37(3): 983 - 998.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
J. E. McGeehan, S. D. Streeter, S.-J. Thresh, N. Ball, R. B. G. Ravelli, and G. G. Kneale
Structural analysis of the genetic switch that regulates the expression of restriction-modification genes
Nucleic Acids Res., August 1, 2008; 36(14): 4778 - 4787.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
I. Mruk and R. M. Blumenthal
Real-time kinetics of restriction-modification gene expression after entry into a new host cell
Nucleic Acids Res., May 1, 2008; 36(8): 2581 - 2593.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
E. Bogdanova, M. Djordjevic, I. Papapanagiotou, T. Heyduk, G. Kneale, and K. Severinov
Transcription regulation of the type II restriction-modification system AhdI
Nucleic Acids Res., March 1, 2008; 36(5): 1429 - 1442.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
I. Mruk, P. Rajesh, and R. M. Blumenthal
Regulatory circuit based on autogenous activation-repression: roles of C-boxes and spacer sequences in control of the PvuII restriction-modification system
Nucleic Acids Res., November 29, 2007; 35(20): 6935 - 6952.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
I. Papapanagiotou, S. D. Streeter, P. D. Cary, and G. G. Kneale
DNA structural deformations in the interaction of the controller protein C.AhdI with its operator sequence
Nucleic Acids Res., April 10, 2007; (2007) gkm129v1.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
E. Semenova, L. Minakhin, E. Bogdanova, M. Nagornykh, A. Vasilov, T. Heyduk, A. Solonin, M. Zakharova, and K. Severinov
Transcription regulation of the EcoRV restriction-modification system
Nucleic Acids Res., December 6, 2005; 33(21): 6942 - 6951.
[Abstract] [Full Text] [PDF]

Home page
 J. Bacteriol.Home page
D. Knowle, R. E. Lintner, Y. M. Touma, and R. M. Blumenthal
Nature of the Promoter Activated by C.PvuII, an Unusual Regulatory Protein Conserved among Restriction-Modification Systems
J. Bacteriol., January 15, 2005; 187(2): 488 - 497.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
S. D. Streeter, I. Papapanagiotou, J. E. McGeehan, and G. G. Kneale
DNA footprinting and biophysical characterization of the controller protein C.AhdI suggests the basis of a genetic switch
Nucleic Acids Res., December 8, 2004; 32(21): 6445 - 6453.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.