Skip Navigation

This Article
Right arrow Full Text Freely available
Right arrow Print PDF (257K) Freely available
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Hodson, J. A.
Right arrow Articles by Forsburg, S. L.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Hodson, J. A.
Right arrow Articles by Forsburg, S. L.
Related Collections
Right arrow Replication
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Nucleic Acids Research, 2003, Vol. 31, No. 21 e134
© 2003 Oxford University Press

Efficient labeling of fission yeast Schizosaccharomyces pombe with thymidine and BUdR

Jeffrey A. Hodson, Julie M. Bailis and Susan L. Forsburg*

Molecular and Cell Biology Laboratory, The Salk Institute, 10010 North Torrey Pines Road, La Jolla, CA 92037, USA

*To whom correspondence should be addressed. Tel: +1 858 453 4100; Fax: +1 858 457 4765; Email: forsburg{at}salk.edu

In this paper we report the construction of a Schizosaccharomyces pombe strain that facilitates analysis of replicating DNA. The strain co-expresses the Herpes simplex virus thymidine kinase gene (hsv-tk) and a human equilibrative nucleoside transporter (hENT1). The double integrant efficiently incorporates 3H-thymidine into nuclear DNA as monitored by scintillation counting. These strains also incorporate the thymidine analog Bromodeoxy uridine (BUdR) into newly replicated DNA, which can be detected by immunofluorescence and flow cytometry. This strain provides a valuable tool for direct study of DNA replication in S.pombe.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Mol. Cell. Biol.Home page
J. M. Bailis, D. D. Luche, T. Hunter, and S. L. Forsburg
Minichromosome Maintenance Proteins Interact with Checkpoint and Recombination Proteins To Promote S-Phase Genome Stability
Mol. Cell. Biol., March 1, 2008; 28(5): 1724 - 1738.
[Abstract] [Full Text] [PDF]

Home page
 GeneticsHome page
N. Nitani, K.-i. Nakamura, C. Nakagawa, H. Masukata, and T. Nakagawa
Regulation of DNA Replication Machinery by Mrc1 in Fission Yeast
Genetics, September 1, 2006; 174(1): 155 - 165.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
T. Silva, R. H. Bradley, Y. Gao, and M. Coue
Xenopus CDC7/DRF1 Complex Is Required for the Initiation of DNA Replication
J. Biol. Chem., April 28, 2006; 281(17): 11569 - 11576.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
J. Postberg, O. Alexandrova, T. Cremer, and H. J. Lipps
Exploiting nuclear duality of ciliates to analyse topological requirements for DNA replication and transcription
J. Cell Sci., September 1, 2005; 118(17): 3973 - 3983.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
Z. You, C. Chahwan, J. Bailis, T. Hunter, and P. Russell
ATM Activation and Its Recruitment to Damaged DNA Require Binding to the C Terminus of Nbs1
Mol. Cell. Biol., July 1, 2005; 25(13): 5363 - 5379.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
D. G. Pankratz and S. L. Forsburg
Meiotic S-Phase Damage Activates Recombination without Checkpoint Arrest
Mol. Biol. Cell, April 1, 2005; 16(4): 1651 - 1660.
[Abstract] [Full Text] [PDF]

Home page
 MicrobiologyHome page
V. H. Vestereng and J. A. Kovacs
Inability of Pneumocystis organisms to incorporate bromodeoxyuridine suggests the absence of a salvage pathway for thymidine
Microbiology, May 1, 2004; 150(5): 1179 - 1182.
[Abstract] [Full Text] [PDF]


Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.