Skip Navigation

This Article
Right arrow Full Text Freely available
Right arrow Print PDF (376K) Freely available
Right arrow Supplementary Material
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (9)
Right arrowRequest Permissions
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Lartigue, C.
Right arrow Articles by Sirand-Pugnet, P.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Lartigue, C.
Right arrow Articles by Sirand-Pugnet, P.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Nucleic Acids Research, 2003, Vol. 31, No. 22 6610-6618
© 2003 Oxford University Press

Article

Host specificity of mollicutes oriC plasmids: functional analysis of replication origin

Carole Lartigue, Alain Blanchard, Joël Renaudin, François Thiaucourt1 and Pascal Sirand-Pugnet*

UMR GDPP, INRA-Université Victor Segalen Bordeaux 2, BP 81, 33883 Villenave d’Ornon Cedex, France and 1 CIRAD-EMVT, Santé Animale, TA30/G, Campus International de Baillarguet, 34398 Montpellier Cedex 5, France

*To whom correspondence should be addressed at INRA, Centre de Recherche de Bordeaux, Institut de Biologie Végétale Moléculaire, 71, avenue Edouard Bourlaux, BP 81, 33883 Villenave d’Ornon Cedex, France. Tel: +33 5 57 12 23 93; Fax: +33 5 57 12 23 69; Email: sirand{at}bordeaux.inra.fr

Recently, artificial oriC plasmids containing the chromosomal dnaA gene and surrounding DnaA box sequences were obtained for the mollicutes Spiroplasma citri and Mycoplasma pulmonis. In order to study the specificity of these plasmids among mollicutes, a set of similar oriC plasmids was developed for three mycoplasmas belonging to the mycoides cluster, Mycoplasma mycoides subsp. mycoides LC (MmmLC), M.mycoides subsp. mycoides SC (MmmSC) and Mycoplasma capricolum subsp. capricolum. Mycoplasmas from the mycoides cluster, S.citri and M.pulmonis were used as recipients for transformation experiments by homologous and heterologous oriC plasmids. All five mollicutes were successfully transformed by homologous plasmids, suggesting that the dnaA gene region represents the functional replication origin of the mollicute chromosomes. However, the ability of mollicutes to replicate heterologous oriC plasmids was found to vary noticeably with the species. For example, the oriC plasmid from M.capricolum did not replicate in the closely related species MmmSC and MmmLC. In contrast, plasmids harbouring the oriC from MmmSC, MmmLC and the more distant species S.citri were all found to replicate in M.capricolum. Our results suggest that the cis-elements present in oriC sequences are not the only determinants of this host specificity.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 MicrobiologyHome page
S.-W. Lee, G. F. Browning, and P. F. Markham
Development of a replicable oriC plasmid for Mycoplasma gallisepticum and Mycoplasma imitans, and gene disruption through homologous recombination in M. gallisepticum
Microbiology, September 1, 2008; 154(9): 2571 - 2580.
[Abstract] [Full Text] [PDF]

Home page
 MicrobiologyHome page
C. Janis, D. Bischof, G. Gourgues, J. Frey, A. Blanchard, and P. Sirand-Pugnet
Unmarked insertional mutagenesis in the bovine pathogen Mycoplasma mycoides subsp. mycoides SC: characterization of a lppQ mutant
Microbiology, August 1, 2008; 154(8): 2427 - 2436.
[Abstract] [Full Text] [PDF]

Home page
 ScienceHome page
C. Lartigue, J. I. Glass, N. Alperovich, R. Pieper, P. P. Parmar, C. A. Hutchison III, H. O. Smith, and J. C. Venter
Genome Transplantation in Bacteria: Changing One Species to Another
Science, August 3, 2007; 317(5838): 632 - 638.
[Abstract] [Full Text] [PDF]

Home page
 J. Bacteriol.Home page
K. S. Wise, M. F. Foecking, K. Roske, Y. J. Lee, Y. M. Lee, A. Madan, and M. J. Calcutt
Distinctive Repertoire of Contingency Genes Conferring Mutation- Based Phase Variation and Combinatorial Expression of Surface Lipoproteins in Mycoplasma capricolum subsp. capricolum of the Mycoplasma mycoides Phylogenetic Cluster
J. Bacteriol., July 1, 2006; 188(13): 4926 - 4941.
[Abstract] [Full Text] [PDF]

Home page
 MicrobiologyHome page
O. Q. Pich, R. Burgos, R. Planell, E. Querol, and J. Pinol
Comparative analysis of antibiotic resistance gene markers in Mycoplasma genitalium: application to studies of the minimal gene complement
Microbiology, February 1, 2006; 152(2): 519 - 527.
[Abstract] [Full Text] [PDF]

Home page
 Appl. Environ. Microbiol.Home page
C. Janis, C. Lartigue, J. Frey, H. Wroblewski, F. Thiaucourt, A. Blanchard, and P. Sirand-Pugnet
Versatile Use of oriC Plasmids for Functional Genomics of Mycoplasma capricolum subsp. capricolum
Appl. Envir. Microbiol., June 1, 2005; 71(6): 2888 - 2893.
[Abstract] [Full Text] [PDF]

Home page
 Genome ResHome page
J. D. Jaffe, N. Stange-Thomann, C. Smith, D. DeCaprio, S. Fisher, J. Butler, S. Calvo, T. Elkins, M. G. FitzGerald, N. Hafez, et al.
The Complete Genome and Proteome of Mycoplasma mobile
Genome Res., August 1, 2004; 14(8): 1447 - 1461.
[Abstract] [Full Text] [PDF]


Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.