Skip Navigation

This Article
Right arrow Full Text Freely available
Right arrow Print PDF (185K) Freely available
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Oberdoerffer, P.
Right arrow Articles by Rajewsky, K.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Oberdoerffer, P.
Right arrow Articles by Rajewsky, K.
Related Collections
Right arrow Recombination
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Nucleic Acids Research, 2003, Vol. 31, No. 22 e140
© 2003 Oxford University Press

Article

Unidirectional Cre-mediated genetic inversion in mice using the mutant loxP pair lox66/lox71

Philipp Oberdoerffer1,2, Kevin L. Otipoby1, Mitsuo Maruyama2,3 and Klaus Rajewsky*,1,2

1 The CBR Institute for Biomedical Research, Harvard Medical School, 200 Longwood Avenue, Boston, MA 02115, USA, 2 Institute for Genetics, University of Cologne, 121 Weyertal, 50932 Cologne, Germany and 3 Laboratory of Pathology, Department of Basic Gerontology, National Institute for Longevity Sciences, 36-3 Gengo, Morioka-Cho, Obu-shi, Aichi 474-8522, Japan

*To whom correspondance should be addressed. Tel: +1 617 278 3067; Fax: +1 617 278 3129; Email: rajewsky{at}cbr.med.harvard.edu

The Cre/loxP recombination system is a commonly used tool to alter the mouse genome in a conditional manner by deletion or inversion of loxP-flanked DNA segments. While Cre-mediated deletion is essentially unidirectional, inversion is reversible and therefore does not allow the stable alteration of gene function in cells that constitutively express Cre. Site-directed mutagenesis yielded a pair of asymmetric loxP sites (lox66 and lox71) that display a favorable forward reaction equilibrium. Here, we demonstrate that lox66/lox71 mediates efficient and predominantly unidirectional inversion of a switch substrate targeted to the mouse genome in combination with either inducible or cell type-specific cre-transgenes in vivo.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Mol. Cell. Biol.Home page
P. Oberdoerffer, C. Kanellopoulou, V. Heissmeyer, C. Paeper, C. Borowski, I. Aifantis, A. Rao, and K. Rajewsky
Efficiency of RNA Interference in the Mouse Hematopoietic System Varies between Cell Types and Developmental Stages
Mol. Cell. Biol., May 15, 2005; 25(10): 3896 - 3905.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.