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Nucleic Acids Research, 2003, Vol. 31, No. 24 7141-7149
© 2003 Oxford University Press

Article

A novel allele of fission yeast rad11 that causes defects in DNA repair and telomere length regulation

Yuuki Ono, Kazunori Tomita, Akira Matsuura2, Takuro Nakagawa3, Hisao Masukata3, Masahiro Uritani, Takashi Ushimaru1 and Masaru Ueno*

Department of Chemistry and 1 Department of Biology, Shizuoka University, 836 OYA, Shizuoka 422-8529, Japan, 2 Department of Geriatric Research, National Institute for Longevity Science, 36-3 Gengo, Morioka, Obu, Aichi 474-8522, Japan and 3 Department of Biology, Graduate School of Science, Osaka University, 1-1, Machikaneyama-cho, Toyonaka, Osaka 560-0043, Japan

*To whom correspondence should be addressed. Tel: +81 54 238 4762; Fax: +81 54 237 3384; Email: scmueno{at}ipc.shizuoka.ac.jp

Replication protein A (RPA) is a heterotrimeric single-stranded DNA-binding protein involved in DNA replication, recombination and repair. In Saccharomyces cerevisiae, several mutants in the RFA1 gene encoding the large subunit of RPA have been isolated and one of the mutants with a missense allele, rfa1-D228Y, shows a synergistic reduction in telomere length when combined with a yku70 mutation. So far, only one mutant allele of the rad11+ gene encoding the large subunit of RPA has been reported in Schizosaccharomyces pombe. To study the role of S.pombe RPA in DNA repair and possibly in telomere maintenance, we constructed a rad11-D223Y mutant, which corresponds to the S.cerevisiae rfa1-D228Y mutant. rad11-D223Y cells were methylmethane sulfonate, hydroxyurea, UV and {gamma}-ray sensitive, suggesting that rad11-D223Y cells have a defect in DNA repair activity. Unlike the S.cerevisiae rfa1-D228Y mutation, the rad11-D223Y mutation itself caused telomere shortening. Moreover, Rad11-Myc bound to telomere in a ChIP assay. These results strongly suggest that RPA is directly involved in telomere maintenance.


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This article has been cited by other articles:


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