Skip Navigation

This Article
Right arrow Full Text Freely available
Right arrow Print PDF (285K) Freely available
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (8)
Right arrowRequest Permissions
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Lee, Y. S.
Right arrow Articles by Kim, J. B.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Lee, Y. S.
Right arrow Articles by Kim, J. B.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Nucleic Acids Research, 2003, Vol. 31, No. 24 7165-7174
© 2003 Oxford University Press

Article

Twist2, a novel ADD1/SREBP1c interacting protein, represses the transcriptional activity of ADD1/SREBP1c

Yun Sok Lee1, Hyoung Ho Lee1, Jiyoung Park1, Eung Jae Yoo1, Carlotta A. Glackin2, Young Il Choi1, Sung Ho Jeon1, Rho Hyun Seong1, Sang Dai Park3 and Jae Bum Kim*,1

1 School of Biological Sciences, Seoul National University, Seoul 151-742, Korea, 2 Division of Molecular Medicine, Beckman Research Institute of the City of Hope, Duarte, CA, USA and 3 International Vaccine Institute, Seoul 151-742, Korea

*To whom correspondence should be addressed. Tel: +82 2 880 5852; Fax: +82 2 878 5852; Email: jaebkim{at}snu.ac.kr

Adipocyte determination and differentiation dependent factor 1 (ADD1)/sterol regulatory element binding protein isoform (SREBP1c) is a key transcription factor in fatty acid metabolism and insulin- dependent gene expression. Although its transcriptional and post-translational regulation has been extensively studied, its regulation by interacting proteins is not well understood. To identify cellular proteins that associate with ADD1/SREBP1c, we employed the yeast two-hybrid system with an adipocyte cDNA library. Using the N-terminal domain of ADD1/SREBP1c as bait, we identified Twist2 (also known as Dermo-1), a basic helix–loop–helix (bHLH) protein, as a novel ADD1/SREBP1c interacting protein. Over-expression of Twist2 strongly repressed the transcriptional activity of ADD1/SREBP1c, primarily by reducing its binding to target sequences. Inhibition of histone deacetylase (HDAC) activity with HDAC inhibitors relieved this repression. Our data suggest that physical interaction between Twist2 and ADD1/SREBP1c attenuates transcriptional activation by ADD1/SREBP1c by inhibiting its binding to DNA, and that this inhibition is at least partly dependent on chromatin modification by HDACs.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Nucleic Acids ResHome page
S. M. Choi, H.-J. Cho, H. Cho, K. H. Kim, J. B. Kim, and H. Park
Stra13/DEC1 and DEC2 inhibit sterol regulatory element binding protein-1c in a hypoxia-inducible factor-dependent mechanism
Nucleic Acids Res., October 5, 2008; (2008) gkn620v1.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Endocrinol. Metab.Home page
G. Bryzgalova, L. Lundholm, N. Portwood, J.-A. Gustafsson, A. Khan, S. Efendic, and K. Dahlman-Wright
Mechanisms of antidiabetogenic and body weight-lowering effects of estrogen in high-fat diet-fed mice
Am J Physiol Endocrinol Metab, October 1, 2008; 295(4): E904 - E912.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Endocrinol.Home page
Y. S. Lee, A Y. Kim, J. W. Choi, M. Kim, S. Yasue, H. J. Son, H. Masuzaki, K. S. Park, and J. B. Kim
Dysregulation of Adipose Glutathione Peroxidase 3 in Obesity Contributes to Local and Systemic Oxidative Stress
Mol. Endocrinol., September 1, 2008; 22(9): 2176 - 2189.
[Abstract] [Full Text] [PDF]

Home page
 Mol Cancer ResHome page
J. H. Suh, E.-Y. Gong, J. B. Kim, I.-K. Lee, H.-S. Choi, and K. Lee
Sterol Regulatory Element-Binding Protein-1c Represses the Transactivation of Androgen Receptor and Androgen-Dependent Growth of Prostatic Cells
Mol. Cancer Res., February 1, 2008; 6(2): 314 - 324.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
Y. S. Lee, D. H. Sohn, D. Han, H.-W. Lee, R. H. Seong, and J. B. Kim
Chromatin Remodeling Complex Interacts with ADD1/SREBP1c To Mediate Insulin-Dependent Regulation of Gene Expression
Mol. Cell. Biol., January 15, 2007; 27(2): 438 - 452.
[Abstract] [Full Text] [PDF]

Home page
 J. Exp. Med.Home page
M. N. Sharif, D. Sosic, C. V. Rothlin, E. Kelly, G. Lemke, E. N. Olson, and L. B. Ivashkiv
Twist mediates suppression of inflammation by type I IFNs and Axl
J. Exp. Med., August 7, 2006; 203(8): 1891 - 1901.
[Abstract] [Full Text] [PDF]

Home page
 DiabetesHome page
Y. S. Lee, W. S. Kim, K. H. Kim, M. J. Yoon, H. J. Cho, Y. Shen, J.-M. Ye, C. H. Lee, W. K. Oh, C. T. Kim, et al.
Berberine, a Natural Plant Product, Activates AMP-Activated Protein Kinase With Beneficial Metabolic Effects in Diabetic and Insulin-Resistant States.
Diabetes, August 1, 2006; 55(8): 2256 - 2264.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
E. J. Yoo, J.-J. Chung, S. S. Choe, K. H. Kim, and J. B. Kim
Down-regulation of Histone Deacetylases Stimulates Adipocyte Differentiation
J. Biol. Chem., March 10, 2006; 281(10): 6608 - 6615.
[Abstract] [Full Text] [PDF]


Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.