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Nucleic Acids Research, 2003, Vol. 31, No. 24 7199-7207
© 2003 Oxford University Press

Article

The linker histone homolog Hho1p from Saccharomyces cerevisiae represents a winged helix–turn–helix fold as determined by NMR spectroscopy

Katsuki Ono1, Osamu Kusano2, Sakurako Shimotakahara2,3, Mitsuhiro Shimizu4, Toshimasa Yamazaki5 and Heisaburo Shindo*,1,2,3

1 School of Life Science and 2 School of Pharmacy, Tokyo University of Pharmacy and Life Science, 1432-1 Horinouchi, Hachioji, Tokyo 192-0392, Japan, 3 Genome Science Center, RIKEN, Tsurumi, Yokohama 230-0045, Japan, 4 Department of Chemistry, Meisei University, 2-1-1 Hodokubo, Hino, Tokyo 191-8506, Japan and 5 Biochemistry Department, National Institute of Agrobiological Sciences, 2-1-2 Kannondai, Tsukuba, Ibaraki 305-8602, Japan

*To whom correspondence should be addressed at School of Pharmacy, Tokyo University of Pharmacy and Life Science, 1432-1 Horinouchi, Hachioji, Tokyo 192-0392, Japan. Tel/Fax: +81 426 76 4537; Email: shindo{at}ps.toyaku.ac.jp

Hho1p is assumed to serve as a linker histone in Saccharomyces cerevisiae and, notably, it possesses two putative globular domains, designated HD1 (residues 41–118) and HD2 (residues 171–252), that are homologous to histone H5 from chicken erythrocytes. We have determined the three-dimensional structure of globular domain HD1 with high precision by heteronuclear magnetic resonance spectroscopy. The structure had a winged helix–turn–helix motif composed of an {alpha}ß{alpha}{alpha}ßß fold and closely resembled the structure of the globular domain of histone H5. Interestingly, the second globular domain, HD2, in Hho1p was unstructured under physiological conditions. Gel mobility assay demonstrated that Hho1p preferentially binds to supercoiled DNA over linearized DNA. Furthermore, NMR analysis of the complex of a deletion mutant protein (residues 1–118) of Hho1p with a linear DNA duplex revealed that four regions within the globular domain HD1 are involved in the DNA binding. The above results suggested that Hho1p possesses properties similar to those of linker histones in higher eukaryotes in terms of the structure and binding preference towards supercoiled DNA.


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 Proc. Natl. Acad. Sci. USAHome page
A. Levy, M. Eyal, G. Hershkovits, M. Salmon-Divon, M. Klutstein, and D. J. Katcoff
Yeast linker histone Hho1p is required for efficient RNA polymerase I processivity and transcriptional silencing at the ribosomal DNA
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