Skip Navigation

This Article
Right arrow Full Text Freely available
Right arrow Print PDF (326K) Freely available
Right arrow Supplementary Material
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (9)
Right arrowRequest Permissions
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Goldschmidt, V.
Right arrow Articles by Marquet, R.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Goldschmidt, V.
Right arrow Articles by Marquet, R.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Nucleic Acids Research, 2003, Vol. 31, No. 3 850-859
© 2003 Oxford University Press

Does the HIV-1 primer activation signal interact with tRNA3Lys during the initiation of reverse transcription?

Valérie Goldschmidt, Chantal Ehresmann, Bernard Ehresmann and Roland Marquet*

UPR 9002 du CNRS affiliée à l’Université Louis Pasteur, Institut de Biologie Moléculaire et Cellulaire, 15 rue René Descartes, 67084 Strasbourg cedex, France

*To whom correspondence should be addressed. Tel: +33 3 88 41 70 91; Fax: +33 3 88 60 22 18; Email: r.marquet{at}ibmc.u-strasbg.fr

Reverse transcription of HIV-1 RNA is primed by a tRNA3Lys molecule bound at the primer binding site (PBS). Complex intermolecular interactions were proposed between tRNA3Lys and the RNA of the HIV-1 Mal isolate. Recently, an alternative interaction was proposed between the T{Psi}C stem of tRNA3Lys and a primer activation signal (PAS) of the Lai and Hxb2 RNAs, suggesting major structural variations in the reverse transcription complex of different HIV-1 strains. Here, we analyzed mutants of the Hxb2 RNA that prevent the interaction between the PAS and tRNA3Lys or/and a complementary sequence in the viral RNA. We compared the kinetics of reverse transcription of the wild type and mutant Hxb2 RNAs, using either tRNA3Lys or an 18mer oligoribonucleotide complementary to the PBS, which cannot interact with the PAS, as primers. We also used chemical probing to test the structure of the mutant and wild type RNAs, as well as the complex formed between the later RNA and tRNA3Lys. These experiments, together with the analysis of long term replication data of mutant viruses obtained by C. Morrow and coworkers (Birmingham, USA) that use alternate tRNAs as primers, strongly suggest that the interaction between the Hxb2 PAS and tRNA3Lys does not exist. Instead, the effects of the vRNA mutations on reverse transcription seem to be linked to incorrect folding of the mutant RNAs.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Nucleic Acids ResHome page
M. Ooms, D. Cupac, T. E. M. Abbink, H. Huthoff, and B. Berkhout
The availability of the primer activation signal (PAS) affects the efficiency of HIV-1 reverse transcription initiation
Nucleic Acids Res., March 12, 2007; 35(5): 1649 - 1659.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
J.-C. Paillart, M. Dettenhofer, X.-f. Yu, C. Ehresmann, B. Ehresmann, and R. Marquet
First Snapshots of the HIV-1 RNA Structure in Infected Cells and in Virions
J. Biol. Chem., November 12, 2004; 279(46): 48397 - 48403.
[Abstract] [Full Text] [PDF]

Home page
 J. Virol.Home page
T. E. M. Abbink, N. Beerens, and B. Berkhout
Forced Selection of a Human Immunodeficiency Virus Type 1 Variant That Uses a Non-Self tRNA Primer for Reverse Transcription: Involvement of Viral RNA Sequences and the Reverse Transcriptase Enzyme
J. Virol., October 1, 2004; 78(19): 10706 - 10714.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
V. Goldschmidt, J.-C. Paillart, M. Rigourd, B. Ehresmann, A.-M. Aubertin, C. Ehresmann, and R. Marquet
Structural Variability of the Initiation Complex of HIV-1 Reverse Transcription
J. Biol. Chem., August 20, 2004; 279(34): 35923 - 35931.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
M. Rigourd, V. Goldschmidt, F. Brule, C. D. Morrow, B. Ehresmann, C. Ehresmann, and R. Marquet
Structure-function relationships of the initiation complex of HIV-1 reverse transcription: the case of mutant viruses using tRNAHis as primer
Nucleic Acids Res., October 1, 2003; 31(19): 5764 - 5775.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
H. Huthoff, K. Bugala, J. Barciszewski, and B. Berkhout
On the importance of the primer activation signal for initiation of tRNAlys3-primed reverse transcription of the HIV-1 RNA genome
Nucleic Acids Res., September 1, 2003; 31(17): 5186 - 5194.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.