Skip Navigation

This Article
Right arrow Full Text Freely available
Right arrow Print PDF (406K) Freely available
Right arrow Supplementary Material
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (42)
Right arrowRequest Permissions
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Hikichi, T.
Right arrow Articles by Ishino, F.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Hikichi, T.
Right arrow Articles by Ishino, F.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Nucleic Acids Research, 2003, Vol. 31, No. 5 1398-1406
© 2003 Oxford University Press

Imprinting regulation of the murine Meg1/Grb10 and human GRB10 genes; roles of brain-specific promoters and mouse-specific CTCF-binding sites

Takafusa Hikichi1,2, Takashi Kohda1,2, Tomoko Kaneko-Ishino2,3 and Fumitoshi Ishino*,1,2

1 Gene Research Center, Tokyo Institute of Technology, 4259 Nagatsuta-cho, Midori-ku, Yokohama 226-8501, Japan, 2 CREST, Japan Science and Technology Corporation (JST), 4-1-8 Hon-machi, Kawaguchi, Saitama 332-0012, Japan and 3 Tokai University, School of Health Sciences, Bohseidai, Isehara, Kanagawa 259-1193, Japan

*To whom correspondence should be addressed at Gene Research Center, Tokyo Institute of Technology, 4259 Nagatsuta-cho, Midori-ku, Yokohama 226-8501, Japan. Tel: +81 45 924 5812; Fax: +81 45 924 5814; Email: fishino{at}bio.titech.ac.jp

The imprinted mouse gene Meg1/Grb10 is expres sed from maternal alleles in almost all tissues and organs, except in the brain, where it is expressed biallelically, and the paternal allele is expressed preferentially in adulthood. In contrast, the human GRB10 gene shows equal biallelic expression in almost all tissues and organs, while it is almost always expressed paternally in the fetal brain. To elucidate the molecular mechanisms of the complex imprinting patterns among the different tissues and organs of humans and mice, we analyzed in detail both the genomic structures and tissue-specific expression profiles of these species. Experiments using 5'-RACE and RT–PCR demonstrated the existence in both humans and mice of novel brain- specific promoters, in which only the paternal allele was active. The promoters were located in the primary differentially methylated regions. Interest ingly, CTCF-binding sites were found only in the mouse promoter region where CTCF showed DNA methylation-sensitive binding activity. Thus, the insulator function of CTCF might cause reciprocal maternal expression of the Meg1/Grb10 gene from another upstream promoter in the mouse, whereas the human upstream promoter is active in both parental alleles due to the lack of the corresponding insulator sequence in this region.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Nucleic Acids ResHome page
J. D. Kim, K. Kang, and J. Kim
YY1's role in DNA methylation of Peg3 and Xist
Nucleic Acids Res., September 1, 2009; 37(17): 5656 - 5664.
[Abstract] [Full Text] [PDF]

Home page
 Hum Mol GenetHome page
D. Monk, P. Arnaud, J. Frost, F. A. Hills, P. Stanier, R. Feil, and G. E. Moore
Reciprocal imprinting of human GRB10 in placental trophoblast and brain: evolutionary conservation of reversed allelic expression
Hum. Mol. Genet., August 15, 2009; 18(16): 3066 - 3074.
[Abstract] [Full Text] [PDF]

Home page
 Hum Mol GenetHome page
H. Shiura, K. Nakamura, T. Hikichi, T. Hino, K. Oda, R. Suzuki-Migishima, T. Kohda, T. Kaneko-Ishino, and F. Ishino
Paternal deletion of Meg1/Grb10 DMR causes maternalization of the Meg1/Grb10 cluster in mouse proximal Chromosome 11 leading to severe pre- and postnatal growth retardation
Hum. Mol. Genet., April 15, 2009; 18(8): 1424 - 1438.
[Abstract] [Full Text] [PDF]

Home page
 Hum Mol GenetHome page
R. Schulz, R. B. McCole, K. Woodfine, A. J. Wood, M. Chahal, D. Monk, G. E. Moore, and R. J. Oakey
Transcript- and tissue-specific imprinting of a tumour suppressor gene
Hum. Mol. Genet., January 1, 2009; 18(1): 118 - 127.
[Abstract] [Full Text] [PDF]

Home page
 DevelopmentHome page
L.-B. Wan, H. Pan, S. Hannenhalli, Y. Cheng, J. Ma, A. Fedoriw, V. Lobanenkov, K. E. Latham, R. M. Schultz, and M. S. Bartolomei
Maternal depletion of CTCF reveals multiple functions during oocyte and preimplantation embryo development
Development, August 15, 2008; 135(16): 2729 - 2738.
[Abstract] [Full Text] [PDF]

Home page
 Genome ResHome page
C. D. Campbell, A. Kirby, J. Nemesh, M. J. Daly, and J. N. Hirschhorn
A survey of allelic imbalance in F1 mice
Genome Res., April 1, 2008; 18(4): 555 - 563.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
T. R. Menheniott, K. Woodfine, R. Schulz, A. J. Wood, D. Monk, A. S. Giraud, H. S. Baldwin, G. E. Moore, and R. J. Oakey
Genomic Imprinting of Dopa decarboxylase in Heart and Reciprocal Allelic Expression with Neighboring Grb10
Mol. Cell. Biol., January 1, 2008; 28(1): 386 - 396.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
L. Wang, B. Balas, C. Y. Christ-Roberts, R. Y. Kim, F. J. Ramos, C. K. Kikani, C. Li, C. Deng, S. Reyna, N. Musi, et al.
Peripheral Disruption of the Grb10 Gene Enhances Insulin Signaling and Sensitivity In Vivo
Mol. Cell. Biol., September 15, 2007; 27(18): 6497 - 6505.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
F. M. Smith, L. J. Holt, A. S. Garfield, M. Charalambous, F. Koumanov, M. Perry, R. Bazzani, S. A. Sheardown, B. D. Hegarty, R. J. Lyons, et al.
Mice with a Disruption of the Imprinted Grb10 Gene Exhibit Altered Body Composition, Glucose Homeostasis, and Insulin Signaling during Postnatal Life
Mol. Cell. Biol., August 15, 2007; 27(16): 5871 - 5886.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
Y. Yamasaki-Ishizaki, T. Kayashima, C. K. Mapendano, H. Soejima, T. Ohta, H. Masuzaki, A. Kinoshita, T. Urano, K.-i. Yoshiura, N. Matsumoto, et al.
Role of DNA Methylation and Histone H3 Lysine 27 Methylation in Tissue-Specific Imprinting of Mouse Grb10
Mol. Cell. Biol., January 15, 2007; 27(2): 732 - 742.
[Abstract] [Full Text] [PDF]

Home page
 Cancer Res.Home page
K. M. Reilly, K. W. Broman, R. T. Bronson, S. Tsang, D. A. Loisel, E. S. Christy, Z. Sun, J. Diehl, D. J. Munroe, and R. G. Tuskan
An Imprinted Locus Epistatically Influences Nstr1 and Nstr2 to Control Resistance to Nerve Sheath Tumors in a Neurofibromatosis Type 1 Mouse Model
Cancer Res., January 1, 2006; 66(1): 62 - 68.
[Abstract] [Full Text] [PDF]

Home page
 Hum Mol GenetHome page
Y. Yamasaki, T. Kayashima, H. Soejima, A. Kinoshita, K.-i. Yoshiura, N. Matsumoto, T. Ohta, T. Urano, H. Masuzaki, T. Ishimaru, et al.
Neuron-specific relaxation of Igf2r imprinting is associated with neuron-specific histone modifications and lack of its antisense transcript Air
Hum. Mol. Genet., September 1, 2005; 14(17): 2511 - 2520.
[Abstract] [Full Text] [PDF]

Home page
 Hum Mol GenetHome page
A. G. West and P. Fraser
Remote control of gene transcription
Hum. Mol. Genet., April 15, 2005; 14(suppl_1): R101 - R111.
[Abstract] [Full Text] [PDF]

Home page
 Genome ResHome page
J. R. Weidman, S. K. Murphy, C. M. Nolan, F. S. Dietrich, and R. L. Jirtle
Phylogenetic Footprint Analysis of IGF2 in Extant Mammals
Genome Res., September 1, 2004; 14(9): 1726 - 1732.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
K. M. Reilly, R. G. Tuskan, E. Christy, D. A. Loisel, J. Ledger, R. T. Bronson, C. D. Smith, S. Tsang, D. J. Munroe, and T. Jacks
Susceptibility to astrocytoma in mice mutant for Nf1 and Trp53 is linked to chromosome 11 and subject to epigenetic effects
PNAS, August 31, 2004; 101(35): 13008 - 13013.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
E. Klenova, A. C. Scott, J. Roberts, S. Shamsuddin, E. A. Lovejoy, S. Bergmann, V. J. Bubb, H.-D. Royer, and J. P. Quinn
YB-1 and CTCF Differentially Regulate the 5-HTT Polymorphic Intron 2 Enhancer Which Predisposes to a Variety of Neurological Disorders
J. Neurosci., June 30, 2004; 24(26): 5966 - 5973.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
Y. Deng, S. Bhattacharya, O. R. Swamy, R. Tandon, Y. Wang, R. Janda, and H. Riedel
Growth Factor Receptor-binding Protein 10 (Grb10) as a Partner of Phosphatidylinositol 3-Kinase in Metabolic Insulin Action
J. Biol. Chem., October 10, 2003; 278(41): 39311 - 39322.
[Abstract] [Full Text] [PDF]

Home page
 Genome ResHome page
R. Ono, H. Shiura, H. Aburatani, T. Kohda, T. Kaneko-Ishino, and F. Ishino
Identification of a Large Novel Imprinted Gene Cluster on Mouse Proximal Chromosome 6
Genome Res., July 1, 2003; 13(7): 1696 - 1705.
[Abstract] [Full Text] [PDF]

Home page
 J BiochemHome page
T. Kaneko-Ishino, T. Kohda, and F. Ishino
The Regulation and Biological Significance of Genomic Imprinting in Mammals
J. Biochem., June 1, 2003; 133(6): 699 - 711.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.