Nucleic Acids Research, 2003, Vol. 31, No. 5 1571-1575
© 2003 Oxford University Press
Transcriptional activation by AP-2
is modulated by the oncogene DEK
Centro de Biología Molecular ‘Severo Ochoa’, CSIC-Universidad Autónoma de Madrid, 28049 Cantoblanco, Madrid, Spain
*To whom correspondence should be addressed. Tel: +34 913 978364; Fax: +34 913 978087; Email: jvazquez{at}cbm.uam.es
The authors wish it to be known that, in their opinion, the first two authors should be regarded as joint First Authors
Cell differentiation and development are highly regulated processes at the transcriptional level. One of the main transcription factors that regulate these processes is AP-2
, a cell-type specific protein required for vertebrate development and embryogenesis. AP-2 also regulates apoptosis and cell-cycle specific events by interacting with the oncogene c-Myc. In searching for novel AP-2- interacting factors, using an affinity chromatography approach, we have observed that oncoprotein DEK interacts with AP-2 in vitro. The existence of an interaction between AP-2 and DEK in cellular cultures was demonstrated by expression of a tagged AP-2 form followed by immunodetection. By transient co-expression experiments using a reporter for APOE promoter activity we have found that DEK stimulates the transactivation activity of AP-2 over APOE promoter. Finally, electrophoretic mobility shift assays suggested that DEK enhances the DNA-binding activity of AP-2. Our data suggest a novel cellular function of DEK as a transcriptional co-activator.
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