Skip Navigation

This Article
Right arrow Full Text Freely available
Right arrow Print PDF (92K) Freely available
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Nicod, J.-C.
Right arrow Articles by Largiadèr, C. R.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Nicod, J.-C.
Right arrow Articles by Largiadèr, C. R.
Related Collections
Right arrow Polymorphism/mutation detection
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Nucleic Acids Research, 2003, Vol. 31, No. 5 e19
© 2003 Oxford University Press

SNPs by AFLP (SBA): a rapid SNP isolation strategy for non-model organisms

Jean-Claude Nicod and Carlo R. Largiadèr*

CMPG, Zoological Institute, University of Bern, Baltzerstrasse 6, CH-3012 Bern, Switzerland

*To whom correspondence should be addressed. Tel: +41 31 631 4513; Fax: +41 31 631 48 88; Email: largiader{at}zoo.unibe.ch
+AJ535561–AJ535589

Despite the great potential of single nucleotide polymorphism (SNP) markers in evolutionary studies, in particular for inferring population genetic parameters, SNP analysis has almost exclusively been limited to humans and ‘genomic model’ organisms, due to the lack of available sequence data in non-model organisms. Here, we describe a rapid and cost effective method to isolate candidate SNPs in non-model organisms. This SNP isolation strategy consists basically in the direct sequencing of amplified fragment length polymorphism bands. In a first application of this method, 10 unique DNA fragments that contained 24 SNPs were discovered in 11.11 kb of sequenced genomic DNA of a non-model species, the brown trout (Salmo trutta).


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 J HeredHome page
S. E. Roden, P. H. Dutton, and P. A. Morin
AFLP Fragment Isolation Technique as a Method to Produce Random Sequences for Single Nucleotide Polymorphism Discovery in the Green Turtle, Chelonia mydas
J. Hered., May 1, 2009; 100(3): 390 - 393.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.