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Nucleic Acids Research, 2003, Vol. 31, No. 7 1955-1961
© 2003 Oxford University Press

Specific SR protein-dependent splicing substrates identified through genomic SELEX

Soyoun Kim, Hua Shi, Dong-ki Lee and John T. Lis

Department of Molecular Biology and Genetics, Cornell University, Ithaca, NY 14853, USA

Dong-ki Lee, Toolgen Inc., Daeduk Biocommunity, Daejeon, 305-729, Korea

The Drosophila pre-mRNA splicing factor B52 (SRp55) is essential for fly development, but splicing of RNAs of specific genes tested previously is normal in B52-null animals, presumably due to partial functional redundancy with other SR proteins. To identify B52-dependent splicing substrates in vivo, we selected genomic sequence fragments whose transcripts bind B52. Almost all of the corresponding genes having a known function encode either transcription factors or components of signal transduction pathways, with the B52- binding fragments located to not only exonic but also intronic regions. Some pre-mRNAs from these genes showed splicing defects in the B52-null mutant. These results indicate that B52 has unique functions in the removal of some introns during development, and plays a critical role in cellular regulatory networks.


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