Skip Navigation

This Article
Right arrow Full Text Freely available
Right arrow Print PDF (257K) Freely available
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Ryffel, G. U.
Right arrow Articles by Senkel, S.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Ryffel, G. U.
Right arrow Articles by Senkel, S.
Related Collections
Right arrow Recombination
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Nucleic Acids Research, 2003, Vol. 31, No. 8 e44
© 2003 Oxford University Press

Tagging muscle cell lineages in development and tail regeneration using Cre recombinase in transgenic Xenopus

Gerhart U. Ryffel, Dagmar Werdien, Gülüzar Turan, Andrea Gerhards, Stefan Gooßes and Sabine Senkel

Institut für Zellbiologie (Tumorforschung), Universitätsklinikum Essen, Hufelandstrasse 55, D-45122 Essen, Germany

*To whom correspondence should be addressed. Tel: +49 201 723 3110; Fax: +49 201 723 5905; Email: gerhart.ryffel{at}uni-essen.de

The use of Cre and FLP recombinases to analyze embryogenesis and organogenesis in Xenopus has not been applied so far. We report on the generation of transgenic Xenopus animals containing a Cre-activated reporter gene cassette expressing blue fluorescent protein that can be switched over to yellow fluorescent protein expression upon Cre-mediated recombination. By injecting Cre mRNA into the two-cell stage embryo we show that Cre-mediated activation of the yellow fluorescent protein gene occurs. In addition, we observe upon injection an extinction of blue fluorescence in animals expressing the transgene and the induction of blue fluorescence in larvae containing a silent reporter gene. By crossing the reporter strains with animals expressing a muscle-specific Cre transgene we obtained an efficient and specific recombination of the reporter gene that leads to yellow fluorescence in myotomes and myofibrils of the developing larvae. Removal of the tail tips of these larvae allows the continuous recording of muscle cell differentiation in the regenerating tail. We detect a dramatic increase in transgene expression at the site of tissue removal in the tail stump. In the regenerated tail, yellow fluorescence is restricted to the myotomes thus excluding transdifferentiation of muscle cells.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 J. Dent. Res.Home page
A.-S. Tseng and M. Levin
Tail Regeneration in Xenopus laevis as a Model for Understanding Tissue Repair
J. Dent. Res., September 1, 2008; 87(9): 806 - 816.
[Abstract] [Full Text] [PDF]

Home page
 Genes Dev.Home page
C. L. Stoick-Cooper, R. T. Moon, and G. Weidinger
Advances in signaling in vertebrate regeneration as a prelude to regenerative medicine
Genes & Dev., June 1, 2007; 21(11): 1292 - 1315.
[Abstract] [Full Text] [PDF]

Home page
 DevelopmentHome page
D. S. Adams, A. Masi, and M. Levin
H+ pump-dependent changes in membrane voltage are an early mechanism necessary and sufficient to induce Xenopus tail regeneration
Development, April 1, 2007; 134(7): 1323 - 1335.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
D. M. Langenau, H. Feng, S. Berghmans, J. P. Kanki, J. L. Kutok, and A. T. Look
Cre/lox-regulated transgenic zebrafish model with conditional myc-induced T cell acute lymphoblastic leukemia
PNAS, April 26, 2005; 102(17): 6068 - 6073.
[Abstract] [Full Text] [PDF]

Home page
 J. Am. Soc. Nephrol.Home page
E. A. Jones
Xenopus: A Prince Among Models for Pronephric Kidney Development
J. Am. Soc. Nephrol., February 1, 2005; 16(2): 313 - 321.
[Abstract] [Full Text] [PDF]

Home page
 J. Am. Soc. Nephrol.Home page
S. Bohn, H. Thomas, G. Turan, S. Ellard, C. Bingham, A. T. Hattersley, and G. U. Ryffel
Distinct Molecular and Morphogenetic Properties of Mutations in the Human HNF1{beta} Gene That Lead to Defective Kidney Development
J. Am. Soc. Nephrol., August 1, 2003; 14(8): 2033 - 2041.
[Abstract] [Full Text] [PDF]


Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.