Skip Navigation

This Article
Right arrow Full Text Freely available
Right arrow Print PDF (402K) Freely available
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (6)
Right arrowRequest Permissions
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Kinoshita, K.
Right arrow Articles by Sogawa, K.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Kinoshita, K.
Right arrow Articles by Sogawa, K.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Published online 9 June 2004

Nucleic Acids Research, 2004, Vol. 32, No. 10 3169-3179
© 2004 Oxford University Press

Altered DNA binding specificity of Arnt by selection of partner bHLH–PAS proteins

Koshi Kinoshita, Yasuo Kikuchi, Yukie Sasakura, Masashi Suzuki1, Yoshiaki Fujii-Kuriyama and Kazuhiro Sogawa*

Department of Biomolecular Sciences, Graduate School of Life Sciences, Tohoku University, Sendai, 980–8578, Japan and 1 AIST-NIBHT CREST Centre of Structural Biology, Higashi 1–1, Tsukuba 305–0046, Japan

*To whom correspondence should be addressed. Tel: +81 22 217 6590; Fax +81 22 217 6594; Email: sogawa{at}mail.tains.tohoku.ac.jp
Present address:
Y. Fujii-Kuriyama, Center for Tsukuba Advanced Reserch Alliance, University Tsukuba, Tsukuba, Ibaraki 305–8577, Japan

Received February 26, 2004; Revised May 3, 2004;; Accepted May 14, 2004

The Ah receptor (AhR) and HLF are transcription factors involved in xenobiotic metabolism and hypoxic response, respectively. AhR and HLF heterodimerize with Arnt as the common partner, and bind to asymmetric E-boxes termed XRE and HRE, respectively. In order to investigate nucleotide preference of the heterodimers, reporter plasmids with oligonucleotides for XREs or HREs with systematic mutations were constructed and their activity was determined. Comparison of the activity revealed that DNA length and nucleotide preference recognized by Arnt subunit in the two heterodimers were largely different between XRE and HRE. We expressed AhR–Arnt and HLF–Arnt in Escherichia coli and used them for DNA binding. The dissociation constant of HLF–Arnt–HRE was 10.4 ± 1.6 nM. Competition activity of mutated XREs or HREs with wild type was consistent with their transcription activity. Bending of XRE and HRE induced by binding of the relevant heterodimers was observed with stronger bending of XRE than of HRE. By deletional and mutational analyses, an alanine and three arginine (Ala 8, Arg 9, Arg 11 and Arg 12) residues in the basic sequence of HLF were found to be indispensable for the transcriptional activity.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Mol. Biol. CellHome page
C.-J. Hu, A. Sataur, L. Wang, H. Chen, and M. C. Simon
The N-Terminal Transactivation Domain Confers Target Gene Specificity of Hypoxia-inducible Factors HIF-1{alpha} and HIF-2{alpha}
Mol. Biol. Cell, November 1, 2007; 18(11): 4528 - 4542.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
L. Jiang and S. T. Crews
Transcriptional Specificity of Drosophila Dysfusion and the Control of Tracheal Fusion Cell Gene Expression
J. Biol. Chem., September 28, 2007; 282(39): 28659 - 28668.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
S. P. Rivera, F. Wang, S. T. Saarikoski, R. T. Taylor, B. Chapman, R. Zhang, and O. Hankinson
A Novel Promoter Element Containing Multiple Overlapping Xenobiotic and Hypoxia Response Elements Mediates Induction of Cytochrome P4502S1 by Both Dioxin and Hypoxia
J. Biol. Chem., April 13, 2007; 282(15): 10881 - 10893.
[Abstract] [Full Text] [PDF]


Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.