Published online 7 July 2004
Nucleic Acids Research, Vol. 32 No. 12 © Oxford University Press 2004; all rights reserved
Demarcating the gene-rich regions of the wheat genome
Washington State University, Pullman, WA 99164, USA and 1 Department of Agronomy and Horticulture, University of Nebraska-Lincoln, Lincoln, NE 68583-0915, USA
* To whom correspondence should be addressed at Department of Crop and Soil Sciences, 277 Johnson Hall, PO Box 646420, Washington State University, Pullman, WA 99164, USA. Tel: +509 335 4666; Fax: +509 335 8674; Email: ksgill{at}wsu.edu
Present addresses: Devinder Sandhu, G302 Agronomy Hall, Department of Agronomy, Iowa State University, Ames, IA-50011-1010, USA
Mustafa Erayman, Agricultural College, Department of Crop Sciences, Mustafa Kemal University, 31034 Hatay, Turkey
Muharrem Dilbirligi, Central Research Institute for Field Crops, Eskisehir yolu, 10 km, Lodumulu/Ankara, Pk: 226, 0642 Ulus/Ankara, Turkey
The authors wish it to be known that, in their opinion, the first four authors should be regarded as joint First Authors
Received January 14, 2004; Revised March 28, 2004; Accepted May 14, 2004
By physically mapping 3025 loci including 252 phenotypically characterized genes and 17 quantitative trait loci (QTLs) relative to 334 deletion breakpoints, we localized the gene-containing fraction to 29% of the wheat genome present as 18 major and 30 minor gene-rich regions (GRRs). The GRRs varied both in gene number and density. The five largest GRRs physically spanning <3% of the genome contained 26% of the wheat genes. Approximate size of the GRRs ranged from 3 to 71 Mb. Recombination mainly occurred in the GRRs. Various GRRs varied as much as 128-fold for gene density and 140-fold for recombination rates. Except for a general suppression in 25–40% of the chromosomal region around centromeres, no correlation of recombination was observed with the gene density, the size, or chromosomal location of GRRs. More than 30% of the wheat genes are in recombination-poor regions thus are inaccessible to map-based cloning.
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