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Nucleic Acids Research 2004 32(13):3957-3966; doi:10.1093/nar/gkh738
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Published online 28 July 2004

Nucleic Acids Research, Vol. 32 No. 13 © Oxford University Press 2004; all rights reserved

Synthesis and processing of tRNA-related SINE transcripts in Arabidopsis thaliana

Thierry Pélissier, Cécile Bousquet-Antonelli, Laurence Lavie and Jean-Marc Deragon*

CNRS UMR 6547 BIOMOVE and GDR 2157, Université Blaise Pascal Clermont-Ferrand II, 63177 Aubière Cedex, France

* To whom correspondence should be addressed. Tel: +33 4 73 40 77 52; Fax: +33 4 73 40 77 77; Email: j-marc.deragon{at}univ-bpclermont.fr
Present address: Laurence Lavie, Department of Human Genetics, Medical School, University of Saar, 66421 Homburg, Germany

Received May 14, 2004; Revised and Accepted July 15, 2004

Despite the ubiquitous distribution of tRNA-related short interspersed elements (SINEs) in eukaryotic species, very little is known about the synthesis and processing of their RNAs. In this work, we have characterized in detail the different RNA populations resulting from the expression of a tRNA-related SINE S1 founder copy in Arabidopsis thaliana. The main population is composed of poly(A)-ending (pa) SINE RNAs, while two minor populations correspond to full-length (fl) or poly(A) minus [small cytoplasmic (sc)] SINE RNAs. Part of the poly(A) minus RNAs is modified by 3'-terminal addition of C or CA nucleotides. All three RNA populations accumulate in the cytoplasm. Using a mutagenesis approach, we show that the poly(A) region and the 3' end unique region, present at the founder locus, are both important for the maturation and the steady-state accumulation of the different S1 RNA populations. The observation that primary SINE transcripts can be post-transcriptionally processed in vivo into a poly(A)-ending species introduces the possibility that this paRNA is used as a retroposition intermediate.


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