Published online 2 August 2004
Nucleic Acids Research, Vol. 32 No. 13 © Oxford University Press 2004; all rights reserved
Single amino acid changes in AspRS reveal alternative routes for expanding its tRNA repertoire in vivo
UPR 9002 SMBMR du CNRS, Institut de Biologie Moléculaire et Cellulaire, 15, rue René Descartes, 67084 Strasbourg, France
* To whom correspondence should be addressed. Tel: +33 3 88 41 70 42; Fax: +33 3 8860 2218; Email: G.Eriani{at}ibmc.u-strasbg.fr
Present address: Sharief Barends, Department of Biochemistry, Leiden Institute of Chemistry, Leiden University, P.O. Box 9502, 2300 RA Leiden, The Netherlands
Received June 8, 2004; Revised and Accepted July 20, 2004
Aminoacyl-tRNA synthetases (aaRSs) are enzymes that are highly specific for their tRNA substrates. Here, we describe the expansion of a class IIb aaRS–tRNA specificity by a genetic selection that involves the use of a modified tRNA displaying an amber anticodon and the argEamber and lacZamber reporters. The study was performed on Escherichia coli aspartyl-tRNA synthetase (AspRS) and amber tRNAAsp. Nine AspRS mutants able to charge the amber tRNAAsp and to suppress the reporter genes were selected from a randomly mutated library. All the mutants exhibited a new amber tRNAAsp specificity in addition to the initial native tRNAAsp. Six mutations were found in the anticodon-binding site located in the N-terminal OB-fold. The strongest suppressor was a mutation of residue Glu-93 that contacts specifically the anticodon nucleotide 34 in the crystal structure. The other mutations in the OB-fold were found at close distance from the anticodon in the so-called loop L45 and strand S1. They concern residues that do not contact tRNAAsp in the native complex. In addition, this study shows that suppressors can carry mutations located far from the anticodon-binding site. One such mutation was found in the synthetase hinge-module where it increases the tRNAAsp-charging rate, and two other mutations were found in the prokaryotic-specific insertion domain and the catalytic core. These mutants seem to act by indirect effects on the tRNA acceptor stem binding and on the conformation of the active site of the enzyme. Altogether, these data suggest the existence of various ways for modifying the mechanism of tRNA discrimination.
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