Published online 19 August 2004
Nucleic Acids Research, Vol. 32 No. 15 © Oxford University Press 2004; all rights reserved
tRNASer(CGA) differentially regulates expression of wild-type and codon-modified papillomavirus L1 genes
Centre for Immunology and Cancer Research, University of Queensland, Princess Alexandra Hospital, Woolloongabba, Queensland 4102, Australia
* To whom correspondence should be addressed. Tel: +61 7 3240 5282; Fax: +61 7 3240 5946; Email: knzhao{at}cicr.uq.edu.au
Present addresses: Mengrong Li, Wenzhou Medical College, PR China
Wei Ming Zhao, School of Medicine, Shandong University, PR China
The authors wish it to be known that, in their opinion, the first three authors should be regarded as joint First Authors
Received May 27, 2004; Revised and Accepted July 20, 2004
Exogenous transfer RNAs (tRNAs) favor translation of bovine papillomavirus 1 wild-type (wt) L1 mRNA in in vitro translation systems (Zhou et al. 1999, J. Virol., 73, 4972–4982). We, therefore, investigated whether papillomavirus (PV) wt L1 protein expression could be enhanced in eukaryotic cells following exogenous tRNA supplementation. Both Chinese hamster ovary (CHO) and Cos1 cells, transfected with PV1 wt L1 genes, effectively transcribed the genes but did not translate them. However, L1 protein translation was demonstrated following co-transfection with the L1 gene and a gene expressing tRNASer(CGA). Cell lines, stably transfected with a bovine papillomavirus 1 (BPV1) wt L1 expression construct, produced L1 protein after the transfection of the tRNASer(CGA) gene, but not following the transfection with basal vectors, suggesting that tRNASer(CGA) gene enhanced wt L1 translation as a result of endogenous tRNA alterations and phosphorylation of translation initiation factors elF4E and elF2
in the tRNASer(CGA) transfected L1 cell lines. The tRNASer(CGA) gene expression significantly reduced translation of L1 proteins expressed from codon-modified (HB) PV L1 genes utilizing mammalian preferred codons, but had variable effects on translation of green fluorescent proteins (GFPs) expressed from six serine GFP variants. The changes of tRNA pools appear to match the codon composition of PV wt and HB L1 genes and serine GFP variants to regulate translation of their mRNAs. These findings demonstrate for the first time in eukaryotic cells that translation of the target genes can be differentially influenced by the provision of a single tRNA expression construct.
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  W. Gu, J. Ding, X. Wang, R. L. de Kluyver, N. A. Saunders, I. H. Frazer, and K.-N. Zhao Generalized substitution of isoencoding codons shortens the duration of papillomavirus L1 protein expression in transiently gene-transfected keratinocytes due to cell differentiation Nucleic Acids Res., July 9, 2007; (2007) gkm496v1. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K.-N. Zhao, W. Gu, N. X. Fang, N. A. Saunders, and I. H. Frazer Gene Codon Composition Determines Differentiation-Dependent Expression of a Viral Capsid Gene in Keratinocytes In Vitro and In Vivo Mol. Cell. Biol., October 1, 2005; 25(19): 8643 - 8655. [Abstract] [Full Text] [PDF]
|
|