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Nucleic Acids Research 2004 32(15):e122; doi:10.1093/nar/gnh121
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Published online 25 August 2004

Nucleic Acids Research, Vol. 32 No. 15 © Oxford University Press 2004; all rights reserved

A ‘one-pot’ assay for the accessibility of DNA in a nucleosome core particle

Chenyi Wu and Andrew Travers*

MRC Laboratory of Molecular Biology, Hills Road, Cambridge CB2 2QH, UK

* To whom correspondence should be addressed. Tel.: +44 1223 402419; Email: aat{at}mrc-lmb.cam.ac.uk

Received July 2, 2004; Revised and Accepted August 12, 2004

The accessibility of nucleosomal DNA to transcription factors and other sequence-specific DNA binding proteins is of importance in the consideration of mechanisms of transcriptional control. Here, we report a simple novel assay which determines this accessibility at eight different rotationally equivalent sites on nucleosomal DNA and shows that linker histones and the chromosomal HMGB proteins, HMG-D and HMG-Z, have opposite effects on the accessibility of nucleosomal DNA. We compare this assay to previously described methods.


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