Skip Navigation

Nucleic Acids Research 2004 32(16):4945-4953; doi:10.1093/nar/gkh811
This Article
Right arrow Full Text Freely available
Right arrow Print PDF (366K) Freely available
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (4)
Right arrowRequest Permissions
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Spiga, M.-G.
Right arrow Articles by D'Urso, G.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Spiga, M.-G.
Right arrow Articles by D'Urso, G.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Published online 23 September 2004

Nucleic Acids Research, Vol. 32 No. 16 © Oxford University Press 2004; all rights reserved

Identification and cloning of two putative subunits of DNA polymerase epsilon in fission yeast

Maria-Grazia Spiga and Gennaro D'Urso*

Department of Biochemistry and Molecular Biology, University of Miami School of Medicine, PO Box 016129, Miami, FL 33101-1019, USA

* To whom correspondence should be addressed. Tel: +1 305 243 3105; Fax: +1 305 243 3064; Email: gdurso{at}miami.edu

Received May 11, 2004; Revised July 20, 2004; Accepted August 18, 2004

DNA polymerase epsilon (Pol {epsilon}) is a multi-subunit enzyme required for the initiation of chromosomal DNA replication. Here, we report the cloning of two fission yeast genes, called dpb3+ and dpb4+ that encode proteins homologous to the two smallest subunits of Pol {epsilon}. Although Dpb4 is not required for cell viability, {Delta}dpb4 mutants are synthetically lethal with mutations in four genes required for DNA replication initiation, cdc20+ (encoding DNA Pol {epsilon}), cut5+ (homologous to DPB11/TopBP1), sna41+ (homologous to CDC45) and cdc21+ (encoding Mcm4, a component of the pre-replicative complex). In contrast to Dpb4, Dpb3 is essential for cell cycle progression. A glutathione S-transferase pull-down assay indicates that Dpb3 physically interacts with both Dpb2 and Dpb4, suggesting that Dpb3 associates with other members of the Pol {epsilon} complex. Depletion of Dpb3 leads to an accumulation of cells in S phase consistent with Dpb3 having a role in DNA replication. In addition, many of the cells have a bi-nucleate or multinucleate phenotype, indicating that cell separation is also inhibited. Finally, we have examined in vivo localization of green fluorescent protein (GFP)-tagged Dpb3 and Dpb4 and found that both proteins are localized to the nucleus consistent with their proposed role in DNA replication. However, in the absence of Dpb3, GFP-Dpb4 appears to be more dispersed throughout the cell, suggesting that Dpb3 may be important in establishing or maintaining normal localization of Dpb4.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 J. Biol. Chem.Home page
P. Walker, D. Doenecke, and J. Kahle
Importin 13 Mediates Nuclear Import of Histone Fold-containing Chromatin Accessibility Complex Heterodimers
J. Biol. Chem., April 24, 2009; 284(17): 11652 - 11662.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
C. C. Pai, I. Garcia, S. W. Wang, S. Cotterill, S. A. MacNeill, and S. E. Kearsey
GINS Inactivation Phenotypes Reveal Two Pathways for Chromatin Association of Replicative {alpha} and {varepsilon} DNA Polymerases in Fission Yeast
Mol. Biol. Cell, February 1, 2009; 20(4): 1213 - 1222.
[Abstract] [Full Text] [PDF]

Home page
 GeneticsHome page
M. Jaszczur, K. Flis, J. Rudzka, J. Kraszewska, M. E. Budd, P. Polaczek, J. L. Campbell, P. Jonczyk, and I. J. Fijalkowska
Dpb2p, a Noncatalytic Subunit of DNA Polymerase {varepsilon}, Contributes to the Fidelity of DNA Replication in Saccharomyces cerevisiae
Genetics, February 1, 2008; 178(2): 633 - 647.
[Abstract] [Full Text] [PDF]

Home page
 GENES CELLSHome page
C. Bauerschmidt, S. Pollok, E. Kremmer, H.-P. Nasheuer, and F. Grosse
Interactions of human Cdc45 with the Mcm2-7 complex, the GINS complex, and DNA polymerases {delta} and {varepsilon} during S phase
Genes Cells, June 1, 2007; 12(6): 745 - 758.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
T. Tsubota, R. Tajima, K. Ode, H. Kubota, N. Fukuhara, T. Kawabata, S. Maki, and H. Maki
Double-stranded DNA Binding, an Unusual Property of DNA Polymerase {epsilon}, Promotes Epigenetic Silencing in Saccharomyces cerevisiae
J. Biol. Chem., October 27, 2006; 281(43): 32898 - 32908.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.