Skip Navigation

Nucleic Acids Research 2004 32(18):5480-5485; doi:10.1093/nar/gkh873
This Article
Right arrow Full Text Freely available
Right arrow Print PDF (200K) Freely available
Right arrow Supplementary Material
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (13)
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Kroeger, K. M.
Right arrow Articles by Greenberg, M. M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Kroeger, K. M.
Right arrow Articles by Greenberg, M. M.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Published online 11 October 2004

Nucleic Acids Research, Vol. 32 No. 18 © Oxford University Press 2004; all rights reserved

A comprehensive comparison of DNA replication past 2-deoxyribose and its tetrahydrofuran analog in Escherichia coli

Kelly M. Kroeger, Myron F. Goodman1 and Marc M. Greenberg*

Department of Chemistry, Johns Hopkins University, 3400 N. Charles Street, Baltimore, MD 21218, USA and 1 Department of Biological Sciences and Chemistry, Hedco Molecular Biology Laboratories, University of Southern California, Los Angeles, CA 90089-1340, USA

* To whom correspondence should be addressed. Tel: +1 410 516 8095; Fax: +1 410 516 7044; Email: mgreenberg{at}jhu.edu

Received August 6, 2004; Revised and Accepted September 17, 2004

Apurinic/apyrimidinic (AP) sites are alkali labile lesions that, when encountered during DNA replication, can block polymerases or potentially result in mutagenic events. Owing to the instability of 2-deoxyribose lesions (AP), a chemically stable tetrahydrofuran analog (F) is often used as a model of abasic sites. A comparison of the two lesions in Saccharomyces cerevisiae revealed that the model lesion and 2-deoxyribose have distinct in vivo effects. Comprehensive comparative analyses of F and AP have not been carried out in Escherichia coli. We conducted a side-by-side investigation of F and AP in E.coli to compare their biological effects and interactions with SOS polymerases. Both lesions were examined in SOS-induced and uninduced cells. Our studies reveal that in uninduced E.coli the effects of individual polymerases in the replication of plasmids containing F or AP are distinct. However, when cells are SOS-induced, the biological effects of F and AP are similar.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 J. Biol. Chem.Home page
W. L. Neeley, S. Delaney, Y. O. Alekseyev, D. F. Jarosz, J. C. Delaney, G. C. Walker, and J. M. Essigmann
DNA Polymerase V Allows Bypass of Toxic Guanine Oxidation Products in Vivo
J. Biol. Chem., April 27, 2007; 282(17): 12741 - 12748.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
Y. W. Kow, G. Bao, B. Minesinger, S. Jinks-Robertson, W. Siede, Y. L. Jiang, and M. M. Greenberg
Mutagenic effects of abasic and oxidized abasic lesions in Saccharomyces cerevisiae
Nucleic Acids Res., October 27, 2005; 33(19): 6196 - 6202.
[Abstract] [Full Text] [PDF]


Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.