Published online 19 October 2004
Nucleic Acids Research, Vol. 32 No. 18 © Oxford University Press 2004; all rights reserved
Structural organization of mRNA complexes with major core mRNP protein YB-1
Institute of Protein Research, Russian Academy of Sciences, Pushchino, Moscow Region 142290, Russia and 1 Faculty of Physics and Faculty of Chemistry of Moscow State University, Moscow 119992, Russia
* To whom correspondence should be addressed. Tel/Fax: +7 095 924 04 93; Email: ovchinn{at}vega.protres.ru
Received July 29, 2004; Revised and Accepted September 27, 2004
YB-1 is a universal major protein of cytoplasmic mRNPs, a member of the family of multifunctional cold shock domain proteins (CSD proteins). Depending on its amount on mRNA, YB-1 stimulates or inhibits mRNA translation. In this study, we have analyzed complexes formed in vitro at various YB-1 to mRNA ratios, including those typical for polysomal (translatable) and free (untranslatable) mRNPs. We have shown that at mRNA saturation with YB-1, this protein alone is sufficient to form mRNPs with the protein/RNA ratio and the sedimentation coefficient typical for natural mRNPs. These complexes are dynamic structures in which the protein can easily migrate from one mRNA molecule to another. Biochemical studies combined with atomic force microscopy and electron microscopy showed that mRNA–YB-1 complexes with a low YB-1/mRNA ratio typical for polysomal mRNPs are incompact; there, YB-1 binds to mRNA as a monomer with its both RNA-binding domains. At a high YB-1/mRNA ratio typical for untranslatable mRNPs, mRNA-bound YB-1 forms multimeric protein complexes where YB-1 binds to mRNA predominantly with its N-terminal part. A multimeric YB-1 comprises about twenty monomeric subunits; its molecular mass is about 700 kDa, and it packs a 600–700 nt mRNA segment on its surface.
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  S. M. Paranjape and E. Harris Y Box-binding Protein-1 Binds to the Dengue Virus 3'-Untranslated Region and Mediates Antiviral Effects J. Biol. Chem., October 19, 2007; 282(42): 30497 - 30508. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 O. M. Alekhina, K. S. Vassilenko, and A. S. Spirin Translation of non-capped mRNAs in a eukaryotic cell-free system: acceleration of initiation rate in the course of polysome formation Nucleic Acids Res., October 8, 2007; 35(19): 6547 - 6559. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. Jonson, J. Vikesaa, A. Krogh, L. K. Nielsen, T. vO. Hansen, R. Borup, A. H. Johnsen, J. Christiansen, and F. C. Nielsen Molecular Composition of IMP1 Ribonucleoprotein Granules Mol. Cell. Proteomics, May 1, 2007; 6(5): 798 - 811. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. J. Tanaka, K. Ogawa, M. Takagi, N. Imamoto, K. Matsumoto, and M. Tsujimoto RAP55, a Cytoplasmic mRNP Component, Represses Translation in Xenopus Oocytes J. Biol. Chem., December 29, 2006; 281(52): 40096 - 40106. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Uchiumi, A. Fotovati, T. Sasaguri, K. Shibahara, T. Shimada, T. Fukuda, T. Nakamura, H. Izumi, T. Tsuzuki, M. Kuwano, et al. YB-1 Is Important for an Early Stage Embryonic Development: NEURAL TUBE FORMATION AND CELL PROLIFERATION J. Biol. Chem., December 29, 2006; 281(52): 40440 - 40449. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Nashchekin, J. Zhao, N. Visa, and B. Daneholt A Novel Ded1-like RNA Helicase Interacts with the Y-box Protein ctYB-1 in Nuclear mRNP Particles and in Polysomes J. Biol. Chem., May 19, 2006; 281(20): 14263 - 14272. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 V. Evdokimova, P. Ruzanov, M. S. Anglesio, A. V. Sorokin, L. P. Ovchinnikov, J. Buckley, T. J. Triche, N. Sonenberg, and P. H. B. Sorensen Akt-Mediated YB-1 Phosphorylation Activates Translation of Silent mRNA Species Mol. Cell. Biol., January 1, 2006; 26(1): 277 - 292. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. I. Young, E. P. Hong, J. C. Castle, J. Crespo-Barreto, A. B. Bowman, M. F. Rose, D. Kang, R. Richman, J. M. Johnson, S. Berget, et al. Inaugural Article: Regulation of RNA splicing by the methylation-dependent transcriptional repressor methyl-CpG binding protein 2 PNAS, December 6, 2005; 102(49): 17551 - 17558. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Zhang, X. Liu, J. G. Cogan, M. D. Fuerst, J. A. Polikandriotis, R. J. Kelm Jr., and A. R. Strauch YB-1 Coordinates Vascular Smooth Muscle {alpha}-Actin Gene Activation by Transforming Growth Factor {beta}1 and Thrombin during Differentiation of Human Pulmonary Myofibroblasts Mol. Biol. Cell, October 1, 2005; 16(10): 4931 - 4940. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. J. Moore From Birth to Death: The Complex Lives of Eukaryotic mRNAs Science, September 2, 2005; 309(5740): 1514 - 1518. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
O. V. Skabkina, D. N. Lyabin, M. A. Skabkin, and L. P. Ovchinnikov YB-1 Autoregulates Translation of Its Own mRNA at or prior to the Step of 40S Ribosomal Subunit Joining Mol. Cell. Biol., April 15, 2005; 25(8): 3317 - 3323. [Abstract] [Full Text] [PDF]
|
|