Skip Navigation

Nucleic Acids Research 2004 32(19):5894-5906; doi:10.1093/nar/gkh922
This Article
Right arrow Full Text Freely available
Right arrow Print PDF (504K) Freely available
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (4)
Right arrowRequest Permissions
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by O'Donnell, A. F.
Right arrow Articles by Singer, R. A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by O'Donnell, A. F.
Right arrow Articles by Singer, R. A.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Published online 1 November 2004

Nucleic Acids Research, Vol. 32 No. 19 © Oxford University Press 2004; all rights reserved

Domain organization of the yeast histone chaperone FACT: the conserved N-terminal domain of FACT subunit Spt16 mediates recovery from replication stress

Allyson F. O'Donnell, Neil K. Brewster, Joelius Kurniawan, Laura V. Minard, Gerald C. Johnston1 and Richard A. Singer*

Department of Biochemistry and Molecular Biology and 1 Department of Microbiology and Immunology, Dalhousie University, Halifax, Nova Scotia, Canada B3H 1X5

* To whom correspondence should be addressed. Tel: +1 902 494 8847; Fax: +1 902 494 1355; Email: Richard.Singer{at}Dal.ca
The authors wish it to be known that, in their opinion, the first two authors should be regarded as joint First Authors

Received August 29, 2004; Revised October 1, 2004; Accepted October 15, 2004

The abundant nuclear complex termed FACT affects several DNA transactions in a chromatin context, including transcription, replication, and repair. Earlier studies of yeast FACT, which indicated the apparent dispensability of conserved sequences at the N terminus of the FACT subunit Cdc68/Spt16, prompted genetic and biochemical studies reported here that suggest the domain organization for Spt16 and the other FACT subunit Pob3, the yeast homolog of the metazoan SSRP1 protein. Our findings suggest that each FACT subunit is a multidomain protein, and that FACT integrity depends on Pob3 interactions with the Spt16 Mid domain. The conserved Spt16 N-terminal domain (NTD) is shown to be without essential function during normal growth, but becomes important under conditions of replication stress. Genetic interactions suggest that some functions carried out by the Spt16 NTD may be partially redundant within FACT.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Proc. Natl. Acad. Sci. USAHome page
T. Stuwe, M. Hothorn, E. Lejeune, V. Rybin, M. Bortfeld, K. Scheffzek, and A. G. Ladurner
The FACT Spt16 "peptidase" domain is a histone H3-H4 binding module
PNAS, July 1, 2008; 105(26): 8884 - 8889.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
A. P. VanDemark, H. Xin, L. McCullough, R. Rawlins, S. Bentley, A. Heroux, D. J. Stillman, C. P. Hill, and T. Formosa
Structural and Functional Analysis of the Spt16p N-terminal Domain Reveals Overlapping Roles of yFACT Subunits
J. Biol. Chem., February 22, 2008; 283(8): 5058 - 5068.
[Abstract] [Full Text] [PDF]

Home page
 GeneticsHome page
D. Biswas, S. Takahata, H. Xin, R. Dutta-Biswas, Y. Yu, T. Formosa, and D. J. Stillman
A Role for Chd1 and Set2 in Negatively Regulating DNA Replication in Saccharomyces cerevisiae
Genetics, February 1, 2008; 178(2): 649 - 659.
[Abstract] [Full Text] [PDF]

Home page
 GeneticsHome page
A. A. Duina, A. Rufiange, J. Bracey, J. Hall, A. Nourani, and F. Winston
Evidence that the Localization of the Elongation Factor Spt16 Across Transcribed Genes Is Dependent Upon Histone H3 Integrity in Saccharomyces cerevisiae
Genetics, September 1, 2007; 177(1): 101 - 112.
[Abstract] [Full Text] [PDF]


Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.